ABCA4 antibody (AA 2250-2263)

Catalog No. ABIN343052

Product Details anti-ABCA4 Antibody

Target: ABCA4 (ATP-Binding Cassette, Sub-Family A (ABC1), Member 4 (ABCA4))

Binding Specificity: AA 2250-2263

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This ABCA4 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF)

Purpose: ABCA4

Sequence: KQQTESHDLP LHPR

Cross-Reactivity: Dog, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Synthetic peptide C-KQQTESHDLPLHPR from the C-terminus of human ABCA4 (NP_000341.2).
Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the Endoplasmic Reticulum of MCF7 and HeLa cells. Recommended concentration: 10μg/ml. This antibody has been successfully used in IF on Mouse, PMID: 32371886.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-ABCA4 antibody (ABIN343052)

McClements, Barnard, Issa, MacLaren: "Assessment of AAV Dual Vector Safety in theAbca4-/- Mouse Model of Stargardt Disease." in: Translational vision science & technology, Vol. 9, Issue 7, pp. 20, (2020) (PubMed).

McClements, Barnard, Singh, Charbel Issa, Jiang, Radu, MacLaren: "An AAV Dual Vector Strategy Ameliorates the Stargardt Phenotype in Adult Abca4-/- Mice." in: Human gene therapy, Vol. 30, Issue 5, pp. 590-600, (2019) (PubMed).

Lenis, Hu, Ng, Jiang, Sarfare, Lloyd, Esposito, Samuel, Jaworski, Bok, Finnemann, Radeke, Redmond, Travis, Radu: "Expression of ABCA4 in the retinal pigment epithelium and its implications for Stargardt macular degeneration." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 115, Issue 47, pp. E11120-E11127, (2019) (PubMed).

Target Details for ABCA4

Target: ABCA4 (ATP-Binding Cassette, Sub-Family A (ABC1), Member 4 (ABCA4))

Alternative Name: ABCA4 (ABCA4 Products)

Synonyms: ABC10 antibody, ABCR antibody, ARMD2 antibody, CORD3 antibody, FFM antibody, RMP antibody, RP19 antibody, STGD antibody, STGD1 antibody, AW050280 antibody, Abc10 antibody, Abcr antibody, D430003I15Rik antibody, RmP antibody, abcr antibody, ffm antibody, rmp antibody, rp19 antibody, stgd antibody, abc10 antibody, armd2 antibody, cord3 antibody, stgd1 antibody, zgc:91823 antibody, ATP binding cassette subfamily A member 4 antibody, ATP-binding cassette, sub-family A (ABC1), member 4 antibody, ATP binding cassette subfamily A member 4 L homeolog antibody, ATP-binding cassette, sub-family A (ABC1), member 4a antibody, ABCA4 antibody, Abca4 antibody, abca4 antibody, abca4.L antibody, abca4a antibody

Background: ABCA4, ATP-binding cassette, sub-family A (ABC1), member 4, ABC10, ABCR, ARMD2, CORD3, DKFZp781N1972, FFM, RMP, RP19, STGD, STGD1, ATP binding cassette transporter, ATP-binding cassette, sub-family A member 4, ATP-binding transporter, retina-specific, ret

Gene ID: 24

NCBI Accession: NP_000341

UniProt: P78363