alpha Fetoprotein antibody

Catalog No. ABIN263679

The Mouse Monoclonal anti-alpha Fetoprotein antibody (Clone 057-11301) (ABIN263679) specifically detects alpha Fetoprotein in EIA. The antibody is reactive with Human samples.

Quick Overview for alpha Fetoprotein antibody (ABIN263679)

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This alpha Fetoprotein antibody is un-conjugated

Application: Enzyme Immunoassay (EIA)

Clone: 057-11301

Product Details anti-alpha Fetoprotein Antibody

Specificity: This antibody reacts to Alpha Fetoprotein (AFP).

Characteristics: Synonyms: Alpha-1-fetoprotein, Alpha-fetoglobulin, HPAFP

Purification: Protein A chromatography

Purity: > 90 % pure (SDS-PAGE)

Immunogen: Human AFP

Isotype: IgG1

Application Details

Application Notes: ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 4.71 mg/mL (OD280nm, E0.1% = 1.4)

Buffer: 10 mM Phosphate, pH 7.4 containing 150 mM Sodium chloride and 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. If aliquoted for long term storage, fill volume should be equal to or greater than 50% of thenominal fill volume of the vial used. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for alpha Fetoprotein

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Background: This is a major plasma protein produced by the yolk sac and the liver during fetal life. alpha Fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha Fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha Fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. alpha Fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha Fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.Synonyms: Alpha-1-fetoprotein, Alpha-fetoglobulin, HPAFP

Gene ID: 174

UniProt: P02771

Pathways: C21-Steroid Hormone Metabolic Process