TNNI3 antibody (AA 148-158)

Catalog No. ABIN264149

The Mouse Monoclonal anti-TNNI3 antibody has been validated for WB, IP, EIA and IHC (fro). It is suitable to detect TNNI3 in samples from Human.

Quick Overview for TNNI3 antibody (AA 148-158) (ABIN264149)

Target: TNNI3 (Cardiac Troponin I (TNNI3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNNI3 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: B159M

Product Details anti-TNNI3 Antibody

Binding Specificity: AA 148-158

Specificity: This antibody reacts with free cardiac troponin I (cTnI). Reacts weakly with cTnI forming complexes with other troponin components. Not affected by phosphorylation. Recognizes an epitope located between amino acid residues 148 and 158 of cTnI. No cross-reactivity with skeletal muscle troponin I.

Characteristics: Synonyms: TNNI3, TNNC1

Purification: Protein A chromatography

Purity: > 90 % pure

Immunogen: Free human cTnI and native human cTn complex

Isotype: IgG1

Application Details

Application Notes: Immunoassay. Immunoprecipitation. Immunohistochemistry. Immunoaffinity purification. Western blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 3.1mg/mL (Sigma protein assay kit)

Buffer: PBS, pH 7.4 containing 0,09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for TNNI3

Target: TNNI3 (Cardiac Troponin I (TNNI3))

Alternative Name: Cardiac Troponin I

Background: Troponin I is part of a heteromeric complex playing an important role in the regulation of skeletal and cardiac muscle contraction. It consists of three subunits, troponin I (TnI), troponin T (TnT) and troponin C (TnC). Each subunit is responsible for part of troponin complex function. TnI inhibits ATPase activity of acto myosin and TnT and TnI are present in cardiac muscles in different forms than in skeletal muscles. Only one tissue specific isoform of TnI is described for cardiac muscle tissue (cTnI) and this is expressed only in myocardium.Synonyms: TNNC1, TNNI3

Gene ID: 7137

UniProt: P19429