CD25 antibody (APC)

Catalog No. ABIN264237

Product Details anti-CD25 Antibody (APC)

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD25 antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Specificity: Identification of human receptor for Interleukin-2 (IL-2R) expressing the 55,000 M.W. surface antigen. This antibody is specific for Interleukin-2 Receptor Lymphocytes.

Characteristics: Synonyms: Interleukin-2 receptor alpha chain, IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, p55, TACantigen

Clone: 1TYV

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry. Monitoring of activated T cells in peripheral blood. Analysis of NK subsets. Study of B cell activation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: PBMC: Add 10 ul of MAB/10e6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2-8°C. Wash twice with PBS and analyze or fix with 0. 5% v/v of paraformaldehyde in PBS andanalyze.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.2 % protein carrier and 0.08 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. Do Not Freeze!
Shelf life: One year from despatch.

Expiry Date: 12 months

Target Details for CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Alternative Name: CD25 / IL2RA (IL2RA Products)

Synonyms: CD25 antibody, IDDM10 antibody, IL2R antibody, TCGFR antibody, IL2RAC antibody, IL-2RA antibody, Il2r antibody, Ly-43 antibody, interleukin 2 receptor subunit alpha antibody, interleukin-2 receptor subunit alpha-like antibody, interleukin 2 receptor, alpha chain antibody, IL2RA antibody, Il2ra antibody, LOC100222957 antibody

Background: The Interleukin 2 Receptor alpha and beta chains, together with the common gamma chain, constitute the high affinity IL2 receptor present on activated T and B cells, thymocyte subset, pre B cells and T regulatory cells. Homodimeric alpha chains result in low affinity receptor, while homodimeric beta chains produce a medium affinity receptor. Normally an integral membrane protein, soluble IL2 Receptor alpha has been isolated and determined to result from extracellular proteolysis. Alternately spliced IL2 Receptor alpha mRNAs have been isolated, but the significance of each is presently unknown.Synonyms: IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, Interleukin-2 receptor alpha chain, TAC antigen, p55

Gene ID: 3559

UniProt: P01589

Pathways: JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation