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LEF1 antibody (HMG Binding Domain)

LEF1 Reactivity: Human, Mouse WB, IF Host: Mouse Monoclonal REMB6 unconjugated
Catalog No. ABIN264380
  • Target See all LEF1 Antibodies
    LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))
    Binding Specificity
    • 11
    • 9
    • 7
    • 6
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    HMG Binding Domain
    Reactivity
    • 81
    • 20
    • 13
    • 7
    • 7
    • 6
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    Human, Mouse
    Host
    • 53
    • 24
    • 4
    • 1
    Mouse
    Clonality
    • 55
    • 27
    Monoclonal
    Conjugate
    • 62
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This LEF1 antibody is un-conjugated
    Application
    • 69
    • 35
    • 13
    • 11
    • 7
    • 6
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF)
    Specificity
    REMB6 recognizes the High Mobility Group DNA domain of LEF-1.
    Characteristics
    Synonyms: Lymphoid enhancer-binding factor 1, LEF-1, T cell-specific transcription factor 1-alpha,TCF1-alpha
    Purification
    Purified
    Immunogen
    Full-length recombinant LEF-1 protein. Genename: LEF1
    Clone
    REMB6
    Isotype
    IgG1
    Top Product
    Discover our top product LEF1 Primary Antibody
  • Application Notes
    Western Blot: 1-5 μg/mLImmunofluorescence on Paraformaldehyde-Fixed Cells: 1 μg/mL (1/1000). Positive Control: Jurkat cell lysate.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Buffer
    PBS, 0.08 % Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store the antibody at -20 °C. Avoid repeated freezing and thawing.
    Shelf life: One year from despatch.
    Expiry Date
    12 months
  • Target
    LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))
    Alternative Name
    LEF1 (LEF1 Products)
    Synonyms
    LEF1 antibody, 3000002B05 antibody, AI451430 antibody, Lef-1 antibody, LEF-1 antibody, TCF10 antibody, TCF1ALPHA antibody, TCF7L3 antibody, lymphoid enhancer binding factor 1 antibody, lymphoid enhancer-binding factor 1 antibody, lymphoid enhancer factor XLEF-1B antibody, LEF1 antibody, lef1 antibody, Lef1 antibody
    Background
    Lymphoid Enhancer Factor-1 is a transcription factor of the High Mobility Group of DNA binding proteins. It is one member of a family of four proteins referred to as LEF/TCF transcription factors (LEF-1, TCF-1, TCF-3 and TCF-4). These factors play a crucial role in WNT/WIngless signaling, a signal transduction cascade that directs cell differentiation. Aberrant activation of the WNT/Wingless pathway is also a root cause in the genesis of certain cancers such as colon cancer, melanoma and breast cancer.LEF-1 is expressed during development in many different differentiating tissues, and in a few tissues after birth. LEF-1 expression is required for proper development of breast, teeth, hair, whiskers and the trigeminal nerve. It is redundant with TCF-1 (for T Cell Factor-1) for correct development of T lymphocytes in the thymus. LEF-1 is a 399 amino acid protein that appears as a cluster of polypeptides ranging in size between 55-57 kD on SDS-PAGE analysis. Alternative splice forms of LEF-1 and other LEF/TCF family members have been identified, but the expression pattern and relative abundance of these alternative forms have not been well studied. They can appear on westerns as smaller immunoreactive forms.Synonyms: LEF-1, Lymphoid enhancer-binding factor 1, T cell-specific transcription factor 1-alpha, TCF1-alpha
    Gene ID
    51176
    UniProt
    Q9UJU2
    Pathways
    WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding
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