Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with asynthetic peptide derived from the Cterminus of the human CYP3A7 protein and mousemyeloma Ag8563 cells.
CYP3A7
Reactivity: Human
WB
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA. Western Blot: 1 - 2 μg/mL. Immunohistochemistry on paraffin sections: 1 - 5 μg/mL. When used in conjunction with CYP3A5, can differentiate between the CYP3A familymembers, 3A5 and 3A7. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Buffer
Phosphate buffered saline with 0.08 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
The antibody can be shipped at ambient temperature. Store (in aliquots) at -20 °C only. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Expiry Date
12 months
Target
CYP3A7
(Cytochrome P450, Family 3, Subfamily A, Polypeptide 7 (CYP3A7))
CP37 antibody, CYPIIIA7 antibody, P450-HFLA antibody, cytochrome P450, family 3, subfamily A, polypeptide 7 antibody, cytochrome P450 family 3 subfamily A member 7 antibody, CYP3A7 antibody
Background
CYP3A genes encode monooxygenases, enzymes which catalyze drug metabolism and the synthesis of cholesterol, steroids and other lipids. CYP3A (cytochrome P450, family 3, subfamily A), the most abundant P450 enzyme in human liver, is responsible for the metabolism of more than 50 % of all clinical drugs. CYP3A members localize in organs that associate with drug disposition, including the liver, gastrointestinal tract and kidney. The CYP3A cluster consists of four genes: CYP3A43, CYP3A4, CYP3A7 and CYP3A5, and two pseudogenes: CYP3A5P1 and CYP3A5P2. The CYP3A cluster maps to gene locus 7q21.3-q22.1.Synonyms: CYPIIIA7, Cytochrome P450 3A7, Cytochrome P450-HFLA