GAL4 antibody (Activation Domain)

Catalog No. ABIN264526

Product Details anti-GAL4 Antibody

Target: GAL4 (LGALS4) (Galectin 4 (LGALS4))

Binding Specificity: Activation Domain

Reactivity: Saccharomyces cerevisiae

Host: Mouse

Clonality: Monoclonal

Conjugate: This GAL4 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Specificity: Recognizes GAL4 derivatives containing the C-terminal activation domain.

Characteristics: Synonyms: GAL81, Regulatory protein GAL4

Purification: Protein A/G Chromatography

Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with full lengthGAL4 expressed in E. Coli and mouse myeloma cells.

Clone: 5C8

Isotype: IgG1

Application Details

Application Notes: Western blot. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), 0.08 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody can be shipped at ambient temperature. Store (in aliquots) at -20 °C only. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for GAL4

Target: GAL4 (LGALS4) (Galectin 4 (LGALS4))

Alternative Name: GAL4 (LGALS4 Products)

Synonyms: LGALS4 antibody, gal4 antibody, l36lbp antibody, MGC86186 antibody, galectin-4 antibody, xgalectin-VIa antibody, gal-4 antibody, L36LBP antibody, L-36 antibody, L36LBI antibody, GAL4 antibody, galectin 4 antibody, lectin, galactoside-binding, soluble, 4 L homeolog antibody, Galectin-4 antibody, lectin, galactose binding, soluble 4 antibody, LGALS4 antibody, lgals4.L antibody, leg4 antibody, Lgals4 antibody

Background: GAL4 is a transcriptional activator which activates genes necessary for galactose catabolism. The DNA binding domain of GAL4 is contained in the first 74 amino acids and contains a zinc finger sequence motif. However, amino acids 1-147 have been shown to bind to DNA in vitro. While GAL4 (1-74) bind DNA specifically, it is with a lower affinity than the GAL4 (1-147). GAL4 (1-147) binds to a specific 17 base pair nucleotide sequence, but alone is insufficient to activate transcription. Either of the two acidic activating regions, activating region I (amino acids 148-238) or activating region II (amino acids 768-881), must be joined to activate transcription.Synonyms: GAL81, Regulatory protein GAL4

Gene ID: 855828

UniProt: P04386