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APAF1 antibody
APAF1
Reactivity: Human
WB, IHC (p), IF, EIA
Host: Rabbit
Polyclonal
unconjugated
Product Details anti-APAF1 Antibody
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Target
See all APAF1 Antibodies
APAF1
(Apoptotic Peptidase Activating Factor 1 (APAF1))
Reactivity
All reactivities for APAF1 antibodies
Human
Host
All hosts for APAF1 antibodies
Rabbit
Clonality
All clonalities for APAF1 antibodies
Polyclonal
Conjugate
All conjugates for APAF1 antibodies
This APAF1 antibody is un-conjugated
Application
All applications for APAF1 antibodies
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Enzyme Immunoassay (EIA)
Specificity
This antibody detects endogenous levels of APAF1 protein (region surrounding His324).
Cross-Reactivity (Details)
Species reactivity (expected):Mouse and Rat. Species reactivity (tested):Human.
Purification
Affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen
Purity
> 95 % pure by SDS-PAGE
Top Product
Discover our top product APAF1 Primary Antibody
Alternatives
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Application Details
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Application Notes
ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Handling
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Concentration
1.0 mg/mL
Buffer
Phosphate buffered saline (PBS), pH 7.2, 0.05 % Sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target Details for APAF1
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Target
APAF1
(Apoptotic Peptidase Activating Factor 1 (APAF1))
Alternative Name
APAF1 (APAF1 Products )
Synonyms
APAF1 antibody, ARK antibody, Apaf-1 antibody, Apaf1 antibody, CG6829 antibody, D-Apaf-1 antibody, DARK antibody, Dapaf-1 antibody, Dapaf-1/HAC-1 antibody, Dark antibody, Dark/Apaf-I antibody, Dark/Dapaf-1/HAC1 antibody, Dark/Hac-1/dApaf-1 antibody, Dark/Hac-1/dApaf1 antibody, Dmel\\CG6829 antibody, Hac-1 antibody, Hac-1/Dark antibody, Hac1 antibody, T1 antibody, anon-53Fa antibody, apaf-1 antibody, apaf1 antibody, arc antibody, ark antibody, dAPAF-1 antibody, dApaf-1 antibody, dApaf-1/DARK/HAC-1 antibody, dApaf1 antibody, dArk antibody, dapaf antibody, dapaf-1 antibody, dapaf-1L antibody, dapaf-1S antibody, dark antibody, dark/dapaf-1/hac-1 antibody, dark/hac-1/dapaf-1 antibody, hac-1 antibody, hac1 antibody, l(2)SH0173 antibody, APAF-1 antibody, CED4 antibody, 6230400I06Rik antibody, Apaf1l antibody, fog antibody, mKIAA0413 antibody, zgc:136543 antibody, Death-associated APAF1-related killer antibody, apoptotic peptidase activating factor 1 L homeolog antibody, apoptotic peptidase activating factor 1 antibody, Dark antibody, apaf1.L antibody, apaf1 antibody, APAF1 antibody, Apaf1 antibody
Background
Studies of apoptosis using the nematode Caenorhabditis elegans as a model system have identified three genes, ced-3, ced-4 and ced-9, as core components in cell death. The Ced-4 protein functions downstream of the apoptotic inhibitor Ced-9 and upstream of Ced-3 to promote cell death. Direct interactions of Ced-4 with Ced-9 and Ced-3 suggest a central role for this protein in the regulation of apoptosis. The mammalian protein AFAP1 contains a domain with homology to Ced-4 and may function in a similar manner by mediating apoptosis downstream of the survival protein Bcl-2 and upstream of the apoptotic inducing caspases. AFAP1 has been shown to bind to cytosolic cytochrome c, and it may mediate the activation of caspase-3 by cytochrome c.Synonyms: Apaf-1, Apoptotic protease-activating factor 1, KIAA0413
Molecular Weight
approx. 38 kDa
Gene ID
317
NCBI Accession
NP_001151
UniProt
O14727
Pathways
p53 Signaling , Apoptosis , Caspase Cascade in Apoptosis , Tube Formation , Positive Regulation of Endopeptidase Activity
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