COPS3
Reactivity: Human, Mouse, Rat
WB, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: 1: 5000approx. 1: 10000. WB: 1: 500approx. 1: 1000. IF: 1: 50approx. 1: 200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Genes belonging to the Jun and Fos oncogene families encode nuclear proteins that are found to be associated with a number of transcriptional complexes. The c-Jun protein is a major component of the transcription factor AP-1, originally shown to mediate phorbol ester tumor promoter (TPA)-induced expression of responsive genes through the TPA-response element (TRE). The Jun proteins form homo- and heterodimers which bind the TRE, but the Fos proteins are active only as heterodimers with any of the Jun proteins. Fos/Jun heterodimers have a much higher affinity for the TRE than Jun homodimers. Ha-Ras augments c-Jun activity and stimulates phosphorylation of its activation domain. The coactivator of Jun, designated JAB1 (for Junactivation domain-binding protein), interacts with c-Jun and Jun D, but not with Jun B or v-Jun. This interaction enhances the transactivating ability of Jun proteins by stabilizing their binding to the TRE.Synonyms: COP9 signalosome complex subunit 3, CSN3, JAB1-containing signalosome subunit 3, SGN3, Signalosome subunit 3