LAMP1 antibody (Lysosomal-Associated Membrane Protein 1)

Details for Product anti-LAMP1 Antibody No. ABIN266767
Target
Reactivity
Human, Mouse, Rat
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96
59
25
15
9
4
3
3
1
1
1
1
Host
Rabbit
93
81
17
1
Clonality
Polyclonal
Conjugate
This LAMP1 antibody is un-conjugated
18
15
15
8
4
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
127
85
64
54
49
41
38
26
21
13
13
6
3
3
2
2
2
2
1
Options
Immunogen Synthetic peptide corresponding to residues C K(407) R S H A G Y Q T I(416) of human LAMP1.
Isotype IgG
Specificity LAMP1 - Lysosome Marker
Cross-Reactivity (Details) Predicted reactivity based on sequence identity: Mouse (100%),rat,(100%).
Purification Immunogen affinity purified
Target
Alternative Name LAMP-1/CD107a (LAMP1 Antibody Abstract)
Background Gene Symbol: LAMP1
Gene ID 3916
UniProt P11279
Pathways Autophagy
Application Notes Western Blot 1:250 - 1:1000, Immunohistochemistry 1:10 - 1:500, Immunocytochemistry/Immunofluorescence 1:10 - 1:500, Immunohistochemistry-Paraffin 1:10 - 1:500ICC usage was reported in scientific literature (PMID: 21631942). WB usage was reported in scientific literature.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer 20 mM Tris ( pH 7.4) and 0.1M Glycine
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.
Images
Image no. 1 for anti-Lysosomal-Associated Membrane Protein 1 (LAMP1) antibody (ABIN266767) Western Blot: LAMP-1/CD107a Antibody [ABIN2667674] - Analysis of 20 ug of HeLa cell l...
Image no. 2 for anti-Lysosomal-Associated Membrane Protein 1 (LAMP1) antibody (ABIN266767) Figure 1 shows Immunofluorescence staining of Lamp1 (red) in HeLa cells using N120-19...
Product cited in: Mittal, Skowyra, Uwase, Tinaztepe, Mehra, Köster, Hanson, Philips: "Mycobacterium tuberculosis Type VII Secretion System Effectors Differentially Impact the ESCRT Endomembrane Damage Response." in: mBio, Vol. 9, Issue 6, 2019 (PubMed). Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC) (Sample species: Mouse (Murine)).

Kevadiya, Woldstad, Ottemann, Dash, Sajja, Lamberty, Morsey, Kocher, Dutta, Bade, Liu, Callen, Fox, Byrareddy, McMillan, Bronich, Edagwa, Boska, Gendelman: "Multimodal Theranostic Nanoformulations Permit Magnetic Resonance Bioimaging of Antiretroviral Drug Particle Tissue-Cell Biodistribution." in: Theranostics, Vol. 8, Issue 1, pp. 256-276, 2018 (PubMed). Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC) (Sample species: Human).

Xie, Zheng, Liu, Qin, Zhao, Cheng, Guo: "BRF1 ameliorates LPS-induced inflammation through autophagy crosstalking with MAPK/ERK signaling." in: Genes & diseases, Vol. 5, Issue 3, pp. 226-234, 2018 (PubMed). Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC) (Sample species: Mouse (Murine)).

Ahmed, Yang, Huang, Chen, Liu, Wang, Nabi, Liu, Chen: "Entosis Acts as a Novel Way within Sertoli Cells to Eliminate Spermatozoa in Seminiferous Tubule." in: Frontiers in physiology, Vol. 8, pp. 361, 2017 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Gouwens, Makoni, Rogers, Nichols: "Amyloid-β42 protofibrils are internalized by microglia more extensively than monomers." in: Brain research, Vol. 1648, Issue Pt A, pp. 485-495, 2017 (PubMed).

Rodriguez, Kaushik, Lapierre, Dever, El-Hage, Nair: "Electro-Magnetic Nano-Particle Bound Beclin1 siRNA Crosses the Blood-Brain Barrier to Attenuate the Inflammatory Effects of HIV-1 Infection in Vitro." in: Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology, 2016 (PubMed). (Sample species: Human).

Sidjanin, Park, Ronchetti, Martins, Jackson: "TBC1D20 mediates autophagy as a key regulator of autophagosome maturation." in: Autophagy, Vol. 12, Issue 10, pp. 1759-1775, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry

Khatter, Raina, Dwivedi, Sindhwani, Bahl, Sharma: "The small GTPase Arl8b regulates assembly of the mammalian HOPS complex on lysosomes." in: Journal of cell science, Vol. 128, Issue 9, pp. 1746-61, 2015 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Dever, Rodriguez, Lapierre, Costin, El-Hage: "Differing roles of autophagy in HIV-associated neurocognitive impairment and encephalitis with implications for morphine co-exposure." in: Frontiers in microbiology, Vol. 6, pp. 653, 2015 (PubMed). Further details: Western Blotting

Case, Chong, Wehrly, Hansen, Child, Hwang, Virgin, Celli: "The Francisella O-antigen mediates survival in the macrophage cytosol via autophagy avoidance." in: Cellular microbiology, Vol. 16, Issue 6, pp. 862-77, 2014 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence

Cai, Xie, Liu, Simone, Caplan, Qin, Naslavsky: "Rapid degradation of the complement regulator, CD59, by a novel inhibitor." in: The Journal of biological chemistry, Vol. 289, Issue 17, pp. 12109-25, 2014 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Puligujja, McMillan, Kendrick, Li, Balkundi, Smith, Veerubhotla, Edagwa, Kabanov, Bronich, Gendelman, Liu: "Macrophage folate receptor-targeted antiretroviral therapy facilitates drug entry, retention, antiretroviral activities and biodistribution for reduction of human immunodeficiency virus infections." in: Nanomedicine : nanotechnology, biology, and medicine, Vol. 9, Issue 8, pp. 1263-73, 2013 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Ejlerskov, Rasmussen, Nielsen, Bergström, Tohyama, Jensen, Vilhardt: "Tubulin polymerization-promoting protein (TPPP/p25α) promotes unconventional secretion of α-synuclein through exophagy by impairing autophagosome-lysosome fusion." in: The Journal of biological chemistry, Vol. 288, Issue 24, pp. 17313-35, 2013 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunocytochemistry,Immunofluorescence

Scharf, Clement, Wu, Morozova, Zanolini, Follenzi, Larocca, Levon, Sutterwala, Rand, Cobelli, Purdue, Hajjar, Santambrogio: "Annexin A2 binds to endosomes following organelle destabilization by particulate wear debris." in: Nature communications, Vol. 3, pp. 755, 2012 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence

Sramkova, Masedunskas, Weigert: "Plasmid DNA is internalized from the apical plasma membrane of the salivary gland epithelium in live animals." in: Histochemistry and cell biology, Vol. 138, Issue 2, pp. 201-13, 2012 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunocytochemistry,Immunofluorescence

Cannizzo, Clement, Morozova, Valdor, Kaushik, Almeida, Follo, Sahu, Cuervo, Macian, Santambrogio: "Age-related oxidative stress compromises endosomal proteostasis." in: Cell reports, Vol. 2, Issue 1, pp. 136-49, 2012 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Kadiu, Nowacek, McMillan, Gendelman: "Macrophage endocytic trafficking of antiretroviral nanoparticles." in: Nanomedicine (London, England), Vol. 6, Issue 6, pp. 975-94, 2011 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Liang, Ouyang, Schneider, Zhang: "Reduction of mutant huntingtin accumulation and toxicity by lysosomal cathepsins D and B in neurons." in: Molecular neurodegeneration, Vol. 6, pp. 37, 2011 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunocytochemistry,Immunofluorescence

Kadiu, Gendelman: "Human immunodeficiency virus type 1 endocytic trafficking through macrophage bridging conduits facilitates spread of infection." in: Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology, Vol. 6, Issue 4, pp. 658-75, 2011 (PubMed).

Ishikawa, Ma, Barber: "STING regulates intracellular DNA-mediated, type I interferon-dependent innate immunity." in: Nature, Vol. 461, Issue 7265, pp. 788-92, 2009 (PubMed). Further details: Immunocytochemistry,Immunofluorescence