Histone 3 antibody (H3K9ac)

Catalog No. ABIN2668419

Product Details anti-Histone 3 Antibody

Target: Histone 3 (H3)

Binding Specificity: H3K9ac

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq)

Purification: None

Immunogen: This Histone H3 acetylLys9 antibody was raised against a peptide including acetyllysine 9 of histone H3.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice:

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage: -20 °C

Storage Comment: Antibodies in solution can be stored at -20 °C for 2 years.

Expiry Date: 6 months

References for anti-Histone 3 antibody (ABIN2668419)

Singh, Johnstone, Martin, Tempera, Kaplan, Denny: "Alterations in nuclear structure promote lupus autoimmunity in a mouse model." in: Disease models & mechanisms, Vol. 9, Issue 8, pp. 885-97, (2016) (PubMed).

Postberg, Kanders, Forcob, Willems, Orth, Hensel, Weil, Wirth, Jenke: "CpG signalling, H2A.Z/H3 acetylation and microRNA-mediated deferred self-attenuation orchestrate foetal NOS3 expression." in: Clinical epigenetics, Vol. 7, Issue 1, pp. 9, (2015) (PubMed).

Castonguay, White, Kagansky, St-Cyr, Castillo, Brugger, White, Bonilla, Spitzer, Earnshaw, Schalch, Ekwall, Tyers, Allshire: "Panspecies small-molecule disruptors of heterochromatin-mediated transcriptional gene silencing." in: Molecular and cellular biology, Vol. 35, Issue 4, pp. 662-74, (2015) (PubMed).

Valdez, Li, Murray, Ji, Liu, Popat, Champlin, Andersson: "Comparison of the cytotoxicity of cladribine and clofarabine when combined with fludarabine and busulfan in AML cells: Enhancement of cytotoxicity with epigenetic modulators." in: Experimental hematology, Vol. 43, Issue 6, pp. 448-61.e2, (2015) (PubMed).

Clifford, Patel, John, Tatler, Mazengarb, Brightling, Knox: "CXCL8 histone H3 acetylation is dysfunctional in airway smooth muscle in asthma: regulation by BET." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 308, Issue 9, pp. L962-72, (2015) (PubMed).

Soyer, Möller, Schotanus, Connolly, Galazka, Freitag, Stukenbrock: "Chromatin analyses of Zymoseptoria tritici: Methods for chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq)." in: Fungal genetics and biology : FG & B, Vol. 79, pp. 63-70, (2015) (PubMed).

Zilio, Codlin, Vashisht, Bitton, Head, Wohlschlegel, Bähler, Boddy: "A novel histone deacetylase complex in the control of transcription and genome stability." in: Molecular and cellular biology, Vol. 34, Issue 18, pp. 3500-14, (2014) (PubMed).

Looney, Zhang, Chen, Lee, Chari, Mao, Pelizzola, Zhang, Lister, Baker, Fernandes, Gaetz, Foshay, Clift, Zhang, Li, Vallender, Wagner, Qin, Michelini, Bugarija, Park, Aryee, Stricker, Zhou, White, Ren et al.: "Systematic mapping of occluded genes by cell fusion reveals prevalence and stability of cis-mediated silencing in somatic cells. ..." in: Genome research, Vol. 24, Issue 2, pp. 267-80, (2014) (PubMed).

Chen, Licon, Otsuka, Pillus, Ideker: "Decoupling epigenetic and genetic effects through systematic analysis of gene position." in: Cell reports, Vol. 3, Issue 1, pp. 128-37, (2013) (PubMed).

Studt, Schmidt, Jahn, Sieber, Connolly, Niehaus, Freitag, Humpf, Tudzynski: "Two histone deacetylases, FfHda1 and FfHda2, are important for Fusarium fujikuroi secondary metabolism and virulence." in: Applied and environmental microbiology, Vol. 79, Issue 24, pp. 7719-34, (2013) (PubMed).

Ingram, Curtis, Silasi-Mansat, Lupu, Griffin: "The NuRD chromatin-remodeling enzyme CHD4 promotes embryonic vascular integrity by transcriptionally regulating extracellular matrix proteolysis." in: PLoS genetics, Vol. 9, Issue 12, pp. e1004031, (2013) (PubMed).

Murata, Narita, Sugimoto, Kawashima, Kanda, Tsurumi: "Contribution of myocyte enhancer factor 2 family transcription factors to BZLF1 expression in Epstein-Barr virus reactivation from latency." in: Journal of virology, Vol. 87, Issue 18, pp. 10148-62, (2013) (PubMed).

Roche, Nasarre, Gemmill, Baldys, Pontis, Korch, Guilhot, Ait-Si-Ali, Drabkin: "Global Decrease of Histone H3K27 Acetylation in ZEB1-Induced Epithelial to Mesenchymal Transition in Lung Cancer Cells." in: Cancers, Vol. 5, Issue 2, pp. 334-56, (2013) (PubMed).

Wiemann, Sieber, von Bargen, Studt, Niehaus, Espino, Huß, Michielse, Albermann, Wagner, Bergner, Connolly, Fischer, Reuter, Kleigrewe, Bald, Wingfield, Ophir, Freeman, Hippler, Smith, Brown, Proctor et al.: "Deciphering the cryptic genome: genome-wide analyses of the rice pathogen Fusarium fujikuroi reveal complex regulation of secondary metabolism and novel metabolites. ..." in: PLoS pathogens, Vol. 9, Issue 6, pp. e1003475, (2013) (PubMed).

Huang, Yin, Sweeney, Raha, Snyder, Lin: "A major epigenetic programming mechanism guided by piRNAs." in: Developmental cell, Vol. 24, Issue 5, pp. 502-16, (2013) (PubMed).

Davis, Curtis, Griffin: "BRG1 promotes COUP-TFII expression and venous specification during embryonic vascular development." in: Development (Cambridge, England), Vol. 140, Issue 6, pp. 1272-81, (2013) (PubMed).

Cie?lik, Hoang, Baranova, Chodaparambil, Kumar, Allison, Xu, Wamsley, Gray, Jones, Mayo, Bekiranov: "Epigenetic coordination of signaling pathways during the epithelial-mesenchymal transition." in: Epigenetics & chromatin, Vol. 6, Issue 1, pp. 28, (2013) (PubMed).

Suzuki, Kuwahara, Tofukuji, Imamura, Kato, Nakayama, Ohara, Yamashita: "A novel small compound SH-2251 suppresses Th2 cell-dependent airway inflammation through selective modulation of chromatin status at the Il5 gene locus." in: PLoS ONE, Vol. 8, Issue 4, pp. e61785, (2013) (PubMed).

Larsson, Ulfhammer, Magnusson, Bergh, Lunke, El-Osta, Medcalf, Svensson, Karlsson, Jern: "Role of histone acetylation in the stimulatory effect of valproic acid on vascular endothelial tissue-type plasminogen activator expression." in: PLoS ONE, Vol. 7, Issue 2, pp. e31573, (2012) (PubMed).

Murata, Kondo, Sugimoto, Kawashima, Saito, Isomura, Kanda, Tsurumi: "Epigenetic histone modification of Epstein-Barr virus BZLF1 promoter during latency and reactivation in Raji cells." in: Journal of virology, Vol. 86, Issue 9, pp. 4752-61, (2012) (PubMed).

Target Details for Histone 3

Target: Histone 3 (H3)

Alternative Name: Histone H3 (H3 Products)

Synonyms: H-3 antibody, histocompatibility 3 antibody, H3 antibody

Molecular Weight: 17 kDa

Gene ID: 3020