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5-Methylcytosine antibody Primary Antibody

DB, MeDIP Host: Rabbit Polyclonal
Pubmed (1)
Catalog No. ABIN2668970
$530.83
Plus shipping costs $45.00 and $24.00 dry ice
100 μL
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  • Target
    Host
    Rabbit
    Clonality
    Polyclonal
    Conjugate
    Un-conjugated
    Application
    Dot Blot (DB), Methylated DNA Immunoprecipitation (MeDIP)
    Purification
    Protein A Chromatography
    Immunogen
    This 5-Methylcytosine (5-mC) antibody was raised against 5-Methyl-cytidine conjugated to KLH and recognizes 5-Methylcytosine.
    Isotype
    IgG
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 μg/μL
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice

    Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

    Storage
    -20 °C
    Storage Comment
    Antibodies in solution can be stored at -20 °C for 2 years.
    Expiry Date
    6 months
  • Murgatroyd, Spengler: "Polycomb binding precedes early-life stress responsive DNA methylation at the Avp enhancer." in: PLoS ONE, Vol. 9, Issue 3, pp. e90277, 2014 (PubMed).

  • Target
    Target Type
    Chemical
    Background
    5-Methylcytosine (5-Methylcytidine) is a modified base that is found in the DNA of plants and vertebrates. DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. The 5-methylcytosine antibody has been developed to discriminate between the modified base and its normal cytosine counterpart, allowing for gene promoter methylation analysis.
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