5-Methylcytosine antibody

Catalog No. ABIN2668970

Product Details

Target:

5-Methylcytosine

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Dot Blot (DB), Methylated DNA Immunoprecipitation (MeDIP)

Purification: Protein A Chromatography

Immunogen: This 5-Methylcytosine (5-mC) antibody was raised against 5-Methyl-cytidine conjugated to KLH and recognizes 5-Methylcytosine.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice:

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage: -20 °C

Storage Comment: Antibodies in solution can be stored at -20 °C for 2 years.

Expiry Date: 6 months

References

Murgatroyd, Spengler: "Polycomb binding precedes early-life stress responsive DNA methylation at the Avp enhancer." in: PLoS ONE, Vol. 9, Issue 3, pp. e90277, 2014 (PubMed).

Target Details

Target:

5-Methylcytosine

Target Type: Chemical

Background: 5-Methylcytosine (5-Methylcytidine) is a modified base that is found in the DNA of plants and vertebrates. DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. The 5-methylcytosine antibody has been developed to discriminate between the modified base and its normal cytosine counterpart, allowing for gene promoter methylation analysis.