5-Hydroxymethylcytosine antibody

Catalog No. ABIN2668980

Product Details anti-5-Hydroxymethylcytosine Antibody

Target: 5-Hydroxymethylcytosine (5-hmC)

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 5-Hydroxymethylcytosine antibody is un-conjugated

Application: Dot Blot (DB), Methylated DNA Immunoprecipitation (MeDIP)

Purification: Protein A Chromatography

Immunogen: This 5-Hydroxymethylcytidine antibody was raised against 5-hydroxymethylcytidine conjugated to KLH and recognizes 5-hydroxymethylcytosine.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice:

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage: -20 °C

Storage Comment: Antibodies in solution can be stored at -20 °C for 2 years.

Expiry Date: 6 months

References for anti-5-Hydroxymethylcytosine antibody (ABIN2668980)

Wiehle, Raddatz, Musch, Dawlaty, Jaenisch, Lyko, Breiling: "Tet1 and Tet2 Protect DNA Methylation Canyons against Hypermethylation." in: Molecular and cellular biology, Vol. 36, Issue 3, pp. 452-61, (2016) (PubMed).

Barazeghi, Gill, Sidhu, Norlén, Dina, Palazzo, Hellman, Stålberg, Westin: "5-Hydroxymethylcytosine discriminates between parathyroid adenoma and carcinoma." in: Clinical epigenetics, Vol. 8, pp. 31, (2016) (PubMed).

Uribe-Lewis, Stark, Carroll, Dunning, Bachman, Ito, Stojic, Halim, Vowler, Lynch, Delatte, de Bony, Colin, Defrance, Krueger, Silva, Ten Hoopen, Ibrahim, Fuks, Murrell: "5-hydroxymethylcytosine marks promoters in colon that resist DNA hypermethylation in cancer." in: Genome biology, Vol. 16, pp. 69, (2015) (PubMed).

Chen, Aksoy, Gonnot, Osteil, Aubry, Hamela, Rognard, Hochard, Voisin, Fontaine, Mure, Afanassieff, Cleroux, Guibert, Chen, Vallot, Acloque, Genthon, Donnadieu, De Vos, Sanlaville, Guérin, Weber et al.: "Reinforcement of STAT3 activity reprogrammes human embryonic stem cells to naive-like pluripotency. ..." in: Nature communications, Vol. 6, pp. 7095, (2015) (PubMed).

Eleftheriou, Pascual, Wheldon, Perry, Abakir, Arora, Johnson, Auer, Ellis, Madhusudan, Ruzov: "5-Carboxylcytosine levels are elevated in human breast cancers and gliomas." in: Clinical epigenetics, Vol. 7, pp. 88, (2015) (PubMed).

Zhao, Yang, Wang, Chong, Yin, Song, Zhao, Li, Huang, Sun, Wu, Jin, Song, Zhu, Jiang, Rendtlew Danielsen, Xu, Yang, Wang: "Redox-active quinones induces genome-wide DNA methylation changes by an iron-mediated and Tet-dependent mechanism." in: Nucleic acids research, Vol. 42, Issue 3, pp. 1593-605, (2014) (PubMed).

Müller, Bauer, Siegl, Rottach, Leonhardt: "TET-mediated oxidation of methylcytosine causes TDG or NEIL glycosylase dependent gene reactivation." in: Nucleic acids research, Vol. 42, Issue 13, pp. 8592-604, (2014) (PubMed).

Hashimoto, Pais, Zhang, Saleh, Fu, Dai, Corrêa, Zheng, Cheng: "Structure of a Naegleria Tet-like dioxygenase in complex with 5-methylcytosine DNA." in: Nature, Vol. 506, Issue 7488, pp. 391-5, (2014) (PubMed).

Colquitt, Allen, Barnea, Lomvardas: "Alteration of genic 5-hydroxymethylcytosine patterning in olfactory neurons correlates with changes in gene expression and cell identity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 36, pp. 14682-7, (2013) (PubMed).

Yamaguchi, Hong, Liu, Inoue, Shen, Zhang, Zhang: "Dynamics of 5-methylcytosine and 5-hydroxymethylcytosine during germ cell reprogramming." in: Cell research, Vol. 23, Issue 3, pp. 329-39, (2013) (PubMed).

Shen, Wu, Diep, Yamaguchi, DAlessio, Fung, Zhang, Zhang: "Genome-wide analysis reveals TET- and TDG-dependent 5-methylcytosine oxidation dynamics." in: Cell, Vol. 153, Issue 3, pp. 692-706, (2013) (PubMed).

Leitch, McEwen, Turp, Encheva, Carroll, Grabole, Mansfield, Nashun, Knezovich, Smith, Surani, Hajkova: "Naive pluripotency is associated with global DNA hypomethylation." in: Nature structural & molecular biology, Vol. 20, Issue 3, pp. 311-6, (2013) (PubMed).

Bocker, Tuorto, Raddatz, Musch, Yang, Xu, Lyko, Breiling: "Hydroxylation of 5-methylcytosine by TET2 maintains the active state of the mammalian HOXA cluster." in: Nature communications, Vol. 3, pp. 818, (2012) (PubMed).

Wu, DAlessio, Ito, Wang, Cui, Zhao, Sun, Zhang: "Genome-wide analysis of 5-hydroxymethylcytosine distribution reveals its dual function in transcriptional regulation in mouse embryonic stem cells." in: Genes & development, Vol. 25, Issue 7, pp. 679-84, (2011) (PubMed).

Li, Cai, Cai, Wang, Zhang, Petersen, Yang, Xu: "Deletion of Tet2 in mice leads to dysregulated hematopoietic stem cells and subsequent development of myeloid malignancies." in: Blood, Vol. 118, Issue 17, pp. 4509-18, (2011) (PubMed).

Target Details for 5-Hydroxymethylcytosine

Target: 5-Hydroxymethylcytosine (5-hmC)

Alternative Name: 5-Hydroxymethylcytosine (5-hmC Products)

Target Type: Chemical