Goat anti-Rabbit IgG (Heavy & Light Chain) Antibody (PE) - Preadsorbed

Catalog No. ABIN129765

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: PE

Application: Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Specificity: IgG (H&L)

Cross-Reactivity: Rabbit

Characteristics: Anti-Rabbit IgG (H&L) phycoerythrin conjugated antibody generated in goat detects specifically rabbit IgG. This secondary phycoerythrin conjugated antibody anti-Rabbit is ideal for investigators who routinely perform titration assays, microscopy and FACS analysis.
Concentration Definition: by absorbance = 82.0 at 565 nm

Purification: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Anti-Rabbit IgG was produced by repeated immunization with rabbit IgG whole molecule in goat.

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Rabbit IgG (H&L) Phycoerythrin Antibody is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 μg of antibody conjugate.  Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.

Flow Cytometry Dilution: 1:100 - 1:250

IF Microscopy Dilution: 1:100 - 1:250

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 0.5 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Product is photosensitive and should be protected from light.

Storage: RT,4 °C

Expiry Date: 12 months

References

Yasunaga, Ohtsubo, Ohno, Saeki, Kurogi, Tanaka-Okamoto, Ishizaki, Shirai, Mihara, Brock, Miyoshi, Takihara: "Scmh1 has E3 ubiquitin ligase activity for geminin and histone H2A and regulates geminin stability directly or indirectly via transcriptional repression of Hoxa9 and Hoxb4." in: Molecular and cellular biology, Vol. 33, Issue 4, pp. 644-60, (2013) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background:

Synonyms: goat anti-Rabbit IgG Antibody, Gt-a-Rabbit Phycoerythrin conjugated, Rabbit IgG (H&L) Antibody in goat, Goat IgG (H&L) Phycoerythrin conjugated Secondary Antibody.

Background: Anti-Rabbit IgG (H&L) phycoerythrin conjugated antibody generated in goat detects specifically rabbit IgG. This secondary phycoerythrin conjugated antibody anti-Rabbit is ideal for investigators who routinely perform titration assays, microscopy and FACS analysis.