Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1) antibody

Details for Product No. ABIN268501
Target
Reactivity
Guinea Pig, Human, Mouse, Rabbit, Rat, Saccharomyces cerevisiae, Sheep
200
112
103
8
7
6
5
5
4
3
3
1
1
1
Host
Mouse
188
25
4
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
22
14
10
8
6
6
5
5
5
5
5
5
3
3
3
3
3
1
1
Application
Blocking Peptide (BP), Chromatin Immunoprecipitation (ChIP), Flow Cytometry (FACS), Gel Shift (GS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Neutralization (Neut), Western Blotting (WB)
156
82
80
51
44
39
30
29
23
12
5
3
3
2
2
1
Options
Immunogen Partially purified rat GR.
Clone BuGR2
Isotype IgG2
Specificity Using enzymatic digestion analysis, has been shown to react with the undigested 97 kDa GR, a 17 kDa DNA-binding trypsin fragment, and a 45 kDa steroid- and DNA-binding chymotrypsin fragment.
Purification Protein A purified
Target
Alternative Name GR/NR3C1 (NR3C1 Antibody Abstract)
Background Gene Symbol: NR3C1
Gene ID 2908
Pathways Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Muscle Cell Differentiation, Regulation of Carbohydrate Metabolic Process
Application Notes Western Blot 5 μg/mL, Chromatin Immunoprecipitation 1:10 - 1:500, Flow Cytometry 1:10 - 1:1000, Immunohistochemistry 1:10 - 1:500, Immunocytochemistry/Immunofluorescence 1:10 - 1:500, Immunoprecipitation 1:10 - 1:500, Immunohistochemistry-Paraffin 5 μg/mL, Gel Super Shift Assays 1:1- 1:100, Blocking/Neutralizing 50 - 200 Molar excessBlocking, ChIP, and ELISA usages were reported in scientific literature.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute with 0.1 mL sterilized water to desired concentration.
Buffer PBS ( pH 7.2)
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.
Images
Image no. 1 for anti-Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1) antibody (ABIN268501) Immunocytochemistry/Immunofluorescence: GR/NR3C1 Antibody (BuGR2) [ABIN268501] - Anal...
Image no. 2 for anti-Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1) antibody (ABIN268501) Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [ABIN268501] - Analysis of Glucocorticoid R...
Image no. 3 for anti-Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1) antibody (ABIN268501) Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [ABIN268501] - Analysis of Glucocorticoid R...
Image no. 4 for anti-Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1) antibody (ABIN268501) Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [ABIN268501] - Analysis of GR in Jurkat cel...
Image no. 5 for anti-Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1) antibody (ABIN268501) Immunocytochemistry/Immunofluorescence: GR/NR3C1 Antibody (BuGR2) [ABIN268501] - Anal...
Image no. 6 for anti-Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1) antibody (ABIN268501) Western Blot: GR/NR3C1 Antibody (BuGR2) [ABIN268501] - Analysis of glucocorticoid rec...
Image no. 7 for anti-Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1) antibody (ABIN268501) Immunocytochemistry/Immunofluorescence: GR/NR3C1 Antibody (BuGR2) [ABIN268501] - Anal...
Product cited in: Taves, Mittelstadt, Presman, Hager, Ashwell: "Single-Cell Resolution and Quantitation of Targeted Glucocorticoid Delivery in the Thymus." in: Cell reports, Vol. 26, Issue 13, pp. 3629-3642.e4, 2019 (PubMed). (Sample species: Mouse (Murine)).

Adams, Smith: "Chronic Gestational Inflammation: Transfer of Maternal Adaptation over Two Generations of Progeny." in: Mediators of inflammation, Vol. 2019, pp. 9160941, 2019 (PubMed). Method employed by authors: Flow Cytometry (FACS), Flow Cytometry (FACS) (Sample species: Mouse (Murine)).

Roh, Park, Jung: "Inhibitory Effects of Prunella vulgaris L. Extract on 11β-HSD1 in Human Skin Cells." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2018, pp. 1762478, 2018 (PubMed). Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC), Immunofluorescence (fixed cells) (IF/ICC) (Sample species: Human).

Rué, Bañez-Coronel, Creus-Muncunill, Giralt, Alcalá-Vida, Mentxaka, Kagerbauer, Zomeño-Abellán, Aranda, Venturi, Pérez-Navarro, Estivill, Martí: "Targeting CAG repeat RNAs reduces Huntington's disease phenotype independently of huntingtin levels." in: The Journal of clinical investigation, Vol. 126, Issue 11, pp. 4319-4330, 2016 (PubMed).

Wu, Pinello, Yissachar, Wischhusen, Yuan, Roberts: "Functionally distinct patterns of nucleosome remodeling at enhancers in glucocorticoid-treated acute lymphoblastic leukemia." in: Epigenetics & chromatin, Vol. 8, pp. 53, 2015 (PubMed). Further details: Chromatin Immunoprecipitation

Schoenfelder, Hiemke, Schmitt: "Behavioural consequences of p-glycoprotein deficiency in mice, with special focus on stress-related mechanisms." in: Journal of neuroendocrinology, Vol. 24, Issue 5, pp. 809-17, 2012 (PubMed). (Sample species: Mouse (Murine)).

Viegas, Vicent, Barañao, Beato, Pecci: "Steroid hormones induce bcl-X gene expression through direct activation of distal promoter P4." in: The Journal of biological chemistry, Vol. 279, Issue 11, pp. 9831-9, 2004 (PubMed).

Harrell, Murphy, Morishima, Chen, Mansfield, Galigniana, Pratt: "Evidence for glucocorticoid receptor transport on microtubules by dynein." in: The Journal of biological chemistry, Vol. 279, Issue 52, pp. 54647-54, 2004 (PubMed).

Lambert, Nordeen: "CBP recruitment and histone acetylation in differential gene induction by glucocorticoids and progestins." in: Molecular endocrinology (Baltimore, Md.), Vol. 17, Issue 6, pp. 1085-94, 2003 (PubMed).

Hutchison, Dittmar, Pratt et al.: "All of the factors required for assembly of the glucocorticoid receptor into a functional heterocomplex with heat shock protein 90 are preassociated in a self-sufficient protein folding structure, a ..." in: The Journal of biological chemistry, Vol. 269, Issue 45, pp. 27894-9, 1994 (PubMed).