His Tag antibody

Details for Product No. ABIN2688142, Supplier: Log in to see
Antigen
Reactivity
Tag
624
28
15
5
4
1
Host
Mouse
413
189
27
23
8
8
3
2
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
58
46
37
17
13
13
10
10
10
9
9
9
8
8
8
7
7
7
5
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Dot Blot (DB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
569
406
164
108
83
81
79
27
13
11
7
7
6
6
5
5
4
4
4
4
2
2
1
1
1
1
1
1
1
1
1
1
Options
Supplier
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Immunogen 6x His synthetic peptide.
Sequence HHHHHH
Clone HIS-H8
Isotype IgG2b
Specificity His-tag fusion protein
Purification IgG fraction
Target Type Tag
Background The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Application Notes ICC/IHC/IF/IP 1/200. WB 1/1000. Other applications are optimized by end users.
Restrictions For Research Use only
Format Liquid
Concentration 1 μg/μL
Buffer PBS, pH 7.4 with 0.05 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Product cited in: Wyroba, Kwaśniak, Miller, Kobyłecki, Osińska: "Site-directed mutagenesis, in vivo electroporation and mass spectrometry in search for determinants of the subcellular targeting of Rab7b paralogue in the model eukaryote Paramecium octaurelia." in: European journal of histochemistry : EJH, Vol. 60, Issue 2, pp. 2612, 2016 (PubMed). Method employed by authors: Confocal Microscopy (CM)

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