VTCN1 antibody

Catalog No. ABIN2688846

Product Details anti-VTCN1 Antibody

Target: VTCN1 (V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This VTCN1 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Characteristics: The MIH43 monoclonal antibody specifically binds to human B7-H4 (B7 family member, H4) that is encoded by the VTCN1 (V-set domain containing T cell activation inhibitor 1) gene. B7-H4 is a type I membrane glycoprotein with a calculated molecular weight of 30.89 kDa. It is also known as VCTN1, B7h.5, B7S1 (B7 superfamily member 1) or B7X. B7-H4 is a newly discovered member of the B7 family of costimulatory proteins. B7-H4 is not constitutively expressed on peripheral tissues. Its expression can be induced on macrophages, dendritic cells, B cells and T cells. By binding to its putative receptor, the function of B7-H4 was initially reported to be a negative regulator of T cell activation, proliferation and differentiation related to cytokine production and cytotoxic effector cell functions. B7-H4 is reportedly overexpressed in a variety tumors. B7-H4 expression by tumor macrophages appears to play a role in suppression antigen-specific T cell mediated immunity. Recent studies indicate that B7-H4 can also be a positive regulator of T cell responses. B7-H4 can be involved innate immunity as well, eg, by inhibiting neutrophil expansion. Flow cytometric analysis of human B7-H4 protein expression on B7-H4-transfected cells. Non-transfected (Left Panel) and B7-H4-transfected (Right Panel) mouse P815 mastocytoma cells were stained with either Purified Mouse IgG1 κ Isotype Control (Cat. No. 554121, dotted line histogram) or Purified Mouse anti-Human B7-H4 (Cat. No. 562506, solid line histogram) followed by PE Goat Anti-Mouse Ig (Multiple Adsorption) (Cat. No. 550589). Flow cytometric fluorescence histograms showing the expression of B7-H4 (or Ig Isotype staining) on non-transfected or transfected cells were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

BD Pharmingen™ Purified Mouse anti-Human B7-H4 - Purified - Clone MIH43 - Isotype Mouse IgG1, κ - Reactivity Hu - 50 μg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Recombinant Human B7-H4 protein

Clone: MIH43

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-VTCN1 antibody (ABIN2688846)

Kryczek, Zou, Rodriguez, Zhu, Wei, Mottram, Brumlik, Cheng, Curiel, Myers, Lackner, Alvarez, Ochoa, Chen, Zou: "B7-H4 expression identifies a novel suppressive macrophage population in human ovarian carcinoma." in: The Journal of experimental medicine, Vol. 203, Issue 4, pp. 871-81, (2006) (PubMed).

Sica, Choi, Zhu, Tamada, Wang, Tamura, Chapoval, Flies, Bajorath, Chen: "B7-H4, a molecule of the B7 family, negatively regulates T cell immunity." in: Immunity, Vol. 18, Issue 6, pp. 849-61, (2003) (PubMed).

Choi, Zhu, Sica, Strome, Cheville, Lau, Zhu, Flies, Tamada, Chen: "Genomic organization and expression analysis of B7-H4, an immune inhibitory molecule of the B7 family." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 9, pp. 4650-4, (2003) (PubMed).

Target Details for VTCN1

Target: VTCN1 (V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1))

Alternative Name: B7-H4 (VTCN1 Products)

Synonyms: B7h4 antibody, B7s1 antibody, B7x antibody, BC032925 antibody, B7-H4 antibody, B7H4 antibody, B7S1 antibody, B7X antibody, B7h.5 antibody, PRO1291 antibody, VCTN1 antibody, RGD1311204 antibody, V set domain containing T cell activation inhibitor 1 L homeolog antibody, V-set domain containing T cell activation inhibitor 1 antibody, V-set domain containing T-cell activation inhibitor 1 antibody, V set domain containing T cell activation inhibitor 1 antibody, vtcn1.L antibody, VTCN1 antibody, vtcn1 antibody, Vtcn1 antibody

Background: Synonyms: VTCN1, VCTN1, B7 family member, H4, B7H4, B7h.5, B7S1, B7X