CD11c antibody (Biotin)

Catalog No. ABIN2688921

Product Details anti-CD11c Antibody (Biotin)

Target: CD11c (ITGAX) (Integrin, alpha X (Complement Component 3 Receptor 4 Subunit) (ITGAX))

Reactivity: Mouse

Host: Armenian Hamster

Clonality: Monoclonal

Conjugate: This CD11c antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Characteristics: The HL3 antibody reacts with the integrin αx chain of gp150, 95 (CD11c/CD18) which is expressed on dendritic cells and CD4- CD8+ intestinal intraepithelial lymphocytes (IEL) and is upregulated on IEL and lymph-node T cells following in vivo activation. CD11c is also found on human NK cells. Although its expression on mouse NK cells is not published, we have detected CD11c on mouse splenic NK cells. Cells of the monocyte/macrophage lineage have been reported to express low levels of CD11c. CD11c plays a role in binding of iC3b. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Expression of CD11c on spleen NK cells. C57BL/6 splenocytes were stained simultaneously with PE-conjugated anti-mouse NK-1.1 mAb PK136 (Cat. No. 557391/553165) and isotype control (left panel) or biotin-conjugated mAb HL3 (right panel), followed by Avidin-FITC (Cat. No. 554057). Flow cytometry was performed on a BD FACScan™ flow cytometry system.

BD Pharmingen™ Biotin Hamster Anti-Mouse CD11c - Biotin - Clone HL3 - Isotype Armenian Hamster IgG1, λ2 - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: C57BL/6 Mouse Intestinal Intraepithelial Lymphocytes

Clone: HL3

Isotype: IgG1 lambda

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing protein stabilizer and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-CD11c antibody (Biotin) (ABIN2688921)

Gao, Liu, Wen, Zhang, Durbin, Liu, Zheng: "Differentiation of monocytic cell clones into CD8 alpha+ dendritic cells (DC) suggests that monocytes can be direct precursors for both CD8 alpha+ and CD8 alpha- DC in the mouse." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 12, pp. 5927-35, (2003) (PubMed).

Pulendran, Lingappa, Kennedy, Smith, Teepe, Rudensky, Maliszewski, Maraskovsky: "Developmental pathways of dendritic cells in vivo: distinct function, phenotype, and localization of dendritic cell subsets in FLT3 ligand-treated mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 5, pp. 2222-31, (1997) (PubMed).

Maraskovsky, Brasel, Teepe, Roux, Lyman, Shortman, McKenna: "Dramatic increase in the numbers of functionally mature dendritic cells in Flt3 ligand-treated mice: multiple dendritic cell subpopulations identified." in: The Journal of experimental medicine, Vol. 184, Issue 5, pp. 1953-62, (1996) (PubMed).

Huleatt, Lefrançois: "Antigen-driven induction of CD11c on intestinal intraepithelial lymphocytes and CD8+ T cells in vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 11, pp. 5684-93, (1995) (PubMed).

Larson, Springer: "Structure and function of leukocyte integrins." in: Immunological reviews, Vol. 114, pp. 181-217, (1990) (PubMed).

Target Details for CD11c

Target: CD11c (ITGAX) (Integrin, alpha X (Complement Component 3 Receptor 4 Subunit) (ITGAX))

Alternative Name: CD11c (ITGAX Products)

Synonyms: CD11C antibody, SLEB6 antibody, RGD1561123 antibody, AI449405 antibody, Cd11c antibody, Cr4 antibody, N418 antibody, integrin subunit alpha X antibody, integrin alpha X antibody, ITGAX antibody, Itgax antibody

Background: Synonyms: Integrin αx chain

Pathways: Complement System, Activated T Cell Proliferation, Integrin Complex