IL7R antibody (Biotin)

Catalog No. ABIN2688948

Product Details anti-IL7R Antibody (Biotin)

Target: IL7R (Interleukin 7 Receptor (IL7R))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL7R antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Characteristics: Monoclonal antibody hIL-7R-M21 reacts with the 60-90 kDa glycoprotein, CD127. CD127 is also known as the IL-7 receptor alpha (IL-7Rα) subunit. The IL-7 receptor complex is a heterodimer composed of CD127 and the common gamma chain (γc, CD132), shared by other cytokine receptors (IL-2R, IL-4R, IL-9R, IL-15R, and IL-21R). CD127 is expressed on thymocytes, T- and B-cell progenitors, mature T cells, and some lymphoid and myeloid cells. In vitro experiments show the expression of CD127 is down-regulated following T cell activation. Studies indicate that the IL-7 Receptor plays an important role in the proliferation and differentiation of mature T cells. Recently, it has been shown that low surface expression of CD127, in combination with intermediate to high surface expression of CD25, the α chain of the IL-2 receptor complex, can distinguish between human regulatory and conventional CD4+ T cells in human adult and cord blood, lymph nodes and thymus. Profile of CD127 (hIL-7R-M21) expression on peripheral blood lymphocytes. Human blood cells were stained with Biotin-anti CD127 (solid line) or Biotin mouse IgG1 isotype control (dashed line, Cat. No. 555747) followed by Streptavidin-PE (Cat. No. 554061). Erythrocytes were lysed using BD PharmLyse™ lysis buffer (Cat. No. 555899). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.

BD Pharmingen™ Biotin Mouse anti-Human CD127 - Biotin - Clone HIL-7R-M21 - Isotype Mouse IgG1, κ - Reactivity Hu - 100 Tests

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Clone: HIL-7R

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 20 μL

Restrictions: For Research Use only

Handling

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-IL7R antibody (Biotin) (ABIN2688948)

Seddiki, Santner-Nanan, Martinson, Zaunders, Sasson, Landay, Solomon, Selby, Alexander, Nanan, Kelleher, Fazekas de St Groth: "Expression of interleukin (IL)-2 and IL-7 receptors discriminates between human regulatory and activated T cells." in: The Journal of experimental medicine, Vol. 203, Issue 7, pp. 1693-700, (2006) (PubMed).

Liu, Putnam, Xu-Yu, Szot, Lee, Zhu, Gottlieb, Kapranov, Gingeras, Fazekas de St Groth, Clayberger, Soper, Ziegler, Bluestone: "CD127 expression inversely correlates with FoxP3 and suppressive function of human CD4+ T reg cells." in: The Journal of experimental medicine, Vol. 203, Issue 7, pp. 1701-11, (2006) (PubMed).

Plum, De Smedt, Leclercq, Verhasselt, Vandekerckhove: "Interleukin-7 is a critical growth factor in early human T-cell development." in: Blood, Vol. 88, Issue 11, pp. 4239-45, (1996) (PubMed).

Hardy, Carmack, Shinton, Kemp, Hayakawa: "Resolution and characterization of pro-B and pre-pro-B cell stages in normal mouse bone marrow." in: The Journal of experimental medicine, Vol. 173, Issue 5, pp. 1213-25, (1991) (PubMed).

Goodwin, Friend, Ziegler, Jerzy, Falk, Gimpel, Cosman, Dower, March, Namen: "Cloning of the human and murine interleukin-7 receptors: demonstration of a soluble form and homology to a new receptor superfamily." in: Cell, Vol. 60, Issue 6, pp. 941-51, (1990) (PubMed).

Namen, Lupton, Hjerrild, Wignall, Mochizuki, Schmierer, Mosley, March, Urdal, Gillis: "Stimulation of B-cell progenitors by cloned murine interleukin-7." in: Nature, Vol. 333, Issue 6173, pp. 571-3, (1988) (PubMed).

Target Details for IL7R

Target: IL7R (Interleukin 7 Receptor (IL7R))

Alternative Name: CD127 (IL7R Products)

Synonyms: IL7R antibody, CD127 antibody, IL-7Ralpha antibody, CDW127 antibody, IL-7R-alpha antibody, IL7RA antibody, ILRA antibody, IL-7RA antibody, interleukin 7 receptor antibody, IL7R antibody, Il7r antibody

Background: Synonyms: IL-7R, IL7R, IL7RA, IL-7Rα, IL-7R-alpha, Interleukin-7 Receptor alpha

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Regulation of Cell Size