IL7R antibody (Biotin)

Catalog No. ABIN2688949

Product Details anti-IL7R Antibody (Biotin)

Target: IL7R (Interleukin 7 Receptor (IL7R))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This IL7R antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Characteristics: The B12-1 monoclonal antibody reacts with CD127, the 65-75- kDa type I transmembrane protein IL- 7Rα. The high affinity IL-7 receptor complex is composed of at least two transmembrane proteins, IL-7Rα and CD132, the common γ chain. CD127 has some sequence homology to the cytokine receptor superfamily (also known as the hematopoietin receptor superfamily). Interaction between IL-7 and its receptor is important for the proliferation of pre-B lymphocytes and can also trigger proliferation of CD4-CD8- immature thymocytes, as well as mature T cells in the periphery. Mice lacking CD127 display profoundly impaired development of the B and T lymphoid cell lineages, but display no obvious non-lymphoid abnormalities.5 IL-7Rα is expressed on common lymphoid progenitors and early stages of B lineage development in the bone marrow, on the earliest thymocyte progenitors and CD4-CD8- double-negative and CD4+ and CD8+ singlepositive thymocytes, and on most peripheral T lymphocytes. Intestinal intraepithelial lymphocytes with low-density γδ TCR upregulate CD127 expression in response to IL-2, which may be secreted by neighboring αβ TCR-bearing T cells. Expression of CD127 in the bone marrow and spleen. C57BL/6 bone marrow leukocytes (Left panels) and splenocytes (Right panels) were stained with either biotinylated rat IgG2a, κ isotype control mAb R35- 95 (Cat. no. 553928, Top panels) or biotinylated B12-1 antibody (Bottom panels) and either FITC-conjugated RA3-6B2 anti-mouse CD45R/B220, Cat. no. 553087/553088, Left panels) or FITC- conjugated 145-2C11 mAb (anti-mouse CD3e, Cat. no. 553061/553062, Right panels), followed by Streptavidin-PE (Cat. no. 554061, all panels). Flow cytometry was performed on a FACScan .

BD Pharmingen™ Biotin Rat Anti-Mouse CD127 - Biotin - Clone B12-1 - Isotype Rat IgG2a, λ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse CD127-human IgG fusion protein

Clone: B12

Isotype: IgG2a lambda

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-IL7R antibody (Biotin) (ABIN2688949)

Akashi, Kondo, Weissman: "Role of interleukin-7 in T-cell development from hematopoietic stem cells." in: Immunological reviews, Vol. 165, pp. 13-28, (1999) (PubMed).

Fujihashi, Kawabata, Hiroi, Yamamoto, McGhee, Nishikawa, Kiyono: "Interleukin 2 (IL-2) and interleukin 7 (IL-7) reciprocally induce IL-7 and IL-2 receptors on gamma delta T-cell receptor-positive intraepithelial lymphocytes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 8, pp. 3613-8, (1996) (PubMed).

Noguchi, Nakamura, Russell, Ziegler, Tsang, Cao, Leonard: "Interleukin-2 receptor gamma chain: a functional component of the interleukin-7 receptor." in: Science (New York, N.Y.), Vol. 262, Issue 5141, pp. 1877-80, (1994) (PubMed).

Peschon, Morrissey, Grabstein, Ramsdell, Maraskovsky, Gliniak, Park, Ziegler, Williams, Ware, Meyer, Davison: "Early lymphocyte expansion is severely impaired in interleukin 7 receptor-deficient mice." in: The Journal of experimental medicine, Vol. 180, Issue 5, pp. 1955-60, (1994) (PubMed).

Faust, Saffran, Toksoz, Williams, Witte: "Distinctive growth requirements and gene expression patterns distinguish progenitor B cells from pre-B cells." in: The Journal of experimental medicine, Vol. 177, Issue 4, pp. 915-23, (1993) (PubMed).

Sudo, Nishikawa, Ohno, Akiyama, Tamakoshi, Yoshida, Nishikawa: "Expression and function of the interleukin 7 receptor in murine lymphocytes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 19, pp. 9125-9, (1993) (PubMed).

Henderson, Narayanan, Collins, Dorshkind: "Status of kappa L chain gene rearrangements and c-kit and IL-7 receptor expression in stromal cell-dependent pre-B cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 6, pp. 1973-9, (1992) (PubMed).

Goodwin, Friend, Ziegler, Jerzy, Falk, Gimpel, Cosman, Dower, March, Namen: "Cloning of the human and murine interleukin-7 receptors: demonstration of a soluble form and homology to a new receptor superfamily." in: Cell, Vol. 60, Issue 6, pp. 941-51, (1990) (PubMed).

Target Details for IL7R

Target: IL7R (Interleukin 7 Receptor (IL7R))

Alternative Name: CD127 (IL7R Products)

Synonyms: IL7R antibody, CD127 antibody, IL-7Ralpha antibody, CDW127 antibody, IL-7R-alpha antibody, IL7RA antibody, ILRA antibody, IL-7RA antibody, interleukin 7 receptor antibody, IL7R antibody, Il7r antibody

Background: Synonyms: IL-7 Receptor α chain

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Regulation of Cell Size