Reacts with 4-1BB, a 30 kDa glycoprotein expressed on activated T cells. 4-1BB can be detected on Con A or PHA-stimulated peripheral blood T cells (CD4+ and CD8+) and on CEM cells (human T-cell leukemia) following 2 day PMA and ionomycin stimulation, but not on resting T cells. 4-1BB is reported to be a participant in T-cell activation, being responsible for rescuing T cells from activation-induced apoptosis, upregulation of Th1-type, T-helper cells, downregulation of Th2-type cytokine production, and induction of cell adhesion to fibronectin. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Profile of Con A-stimulated peripheral blood mononuclear cells (PBMC) analyzed by flow cytometry. Second step staining with Cat. No. 555988.
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C
Storage Comment
Store undiluted at 4°C.
Garni-Wagner, Lee, Kim, Wilde, Kang, Kwon: "4-1BB is expressed on CD45RAhiROhi transitional T cell in humans." in: Cellular immunology, Vol. 169, Issue 1, pp. 91-8, (1996) (PubMed).
Zhou, Kim, Hurtado, Lee, Kim, Pollok, Kwon: "Characterization of human homologue of 4-1BB and its ligand." in: Immunology letters, Vol. 45, Issue 1-2, pp. 67-73, (1995) (PubMed).
Hurtado, Kim, Pollok, Lee, Kwon: "Potential role of 4-1BB in T cell activation. Comparison with the costimulatory molecule CD28." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 7, pp. 3360-7, (1995) (PubMed).
Target
CD137 (TNFRSF9)
(Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9))