CD21 antibody (Biotin)

Catalog No. ABIN2689089

Product Details anti-CD21 Antibody (Biotin)

Target: CD21 (CR2) (Complement Component (3d/Epstein Barr Virus) Receptor 2 (CR2))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD21 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Characteristics: The 7G6 antibody recognizes an epitope shared by 145-150- kDa and 190- kDa complement receptor proteins, originally designated CR2 (CD21) and CR1 (CD35), respectively. In the mouse, CD21 and CD35 are expressed on the majority of peripheral B lymphocytes, on the majority of resident peritoneal macrophages and mast cells, on peripheral blood granulocytes after treatment with N-formyl-Met-Leu-Phe, and on follicular dendritic cells, but not on thymocytes, T cells, erythrocytes, or platelets. CD21 is a ligand-binding component of the CD19/CD21/CD81 signal-transduction complex associated with the antigen receptor on B lymphocytes. CD21/CD35 also co-localizes with CD19 on the surface of peritoneal mast cells. Cr2null mice display impaired inflammatory and humoral immune responses in vivo. The 7G6 mAb has been reported to inhibit rosette formation by C3d-bearing sheep erythrocytes, to block the complement dependent trapping of immune complexes by follicular dendritic cells, and to down-regulate mouse CD21/CD35 expression upon in vivo application, thus inhibiting primary antibody responses to immunization. Co-stimulation of B-cell differentiation via Sepharose-coupled 7G6 antibody has also been observed. The 7G6 mAb recognizes an epitope on CD35 distinct from the epitope recognized by anti-mouse CD35, clone 8C12 (Cat. No. 558768, for the purified antibody), and it does not block binding of 8C12 mAb to mouse CD35. Multicolor flow cytometric analysis of CD21/CD35 expression on BALB/c mouse spleen. Splenocytes were stained simultaneously with FITC Rat Anti-Mouse CD45R/B220 antibody (Cat. No. 553087/553088) and with either Biotin Rat IgG2b, κ Isotype Control (Cat. No. 553987, Left Panel) or Biotin Rat anti-Mouse CD21/CD35 (Cat. No. 562796, Right Panel), followed by Streptavidin-PE (Cat. No. 554061). Two-color flow cytometric dot plots show the correlated expression patterns of CD21/CD35 (or Ig isotype control staining) versus CD45R for gated events with the forward and side light-scatter characteristics of viable spleen cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

BD Pharmingen™ Biotin Rat Anti-Mouse CD21/CD35 - Biotin - Clone 7G6 - Isotype Rat IgG2b, κ - Reactivity Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Clone: 7G6

Isotype: IgG2b kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CD21 antibody (Biotin) (ABIN2689089)

Fagarasan, Muramatsu, Suzuki, Nagaoka, Hiai, Honjo: "Critical roles of activation-induced cytidine deaminase in the homeostasis of gut flora." in: Science (New York, N.Y.), Vol. 298, Issue 5597, pp. 1424-7, (2002) (PubMed).

Cariappa, Tang, Parng, Nebelitskiy, Carroll, Georgopoulos, Pillai: "The follicular versus marginal zone B lymphocyte cell fate decision is regulated by Aiolos, Btk, and CD21." in: Immunity, Vol. 14, Issue 5, pp. 603-15, (2001) (PubMed).

Gommerman, Oh, Zhou, Tedder, Maurer, Galli, Carroll: "A role for CD21/CD35 and CD19 in responses to acute septic peritonitis: a potential mechanism for mast cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 12, pp. 6915-21, (2000) (PubMed).

Oliver, Martin, Kearney: "IgMhighCD21high lymphocytes enriched in the splenic marginal zone generate effector cells more rapidly than the bulk of follicular B cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 12, pp. 7198-207, (1999) (PubMed).

Fischer, Goerg, Shen, Prodeus, Goodnow, Kelsoe, Carroll: "Dependence of germinal center B cells on expression of CD21/CD35 for survival." in: Science (New York, N.Y.), Vol. 280, Issue 5363, pp. 582-5, (1998) (PubMed).

Hu, Martin, Weis, Weis: "Expression of the murine CD21 gene is regulated by promoter and intronic sequences." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 10, pp. 4758-68, (1997) (PubMed).

Oliver, Martin, Gartland, Carter, Kearney: "Marginal zone B cells exhibit unique activation, proliferative and immunoglobulin secretory responses." in: European journal of immunology, Vol. 27, Issue 9, pp. 2366-74, (1997) (PubMed).

Ahearn, Fischer, Croix, Goerg, Ma, Xia, Zhou, Howard, Rothstein, Carroll: "Disruption of the Cr2 locus results in a reduction in B-1a cells and in an impaired B cell response to T-dependent antigen." in: Immunity, Vol. 4, Issue 3, pp. 251-62, (1996) (PubMed).

Axcrona, Gray, Leanderson: "Regulation of B cell growth and differentiation via CD21 and CD40." in: European journal of immunology, Vol. 26, Issue 9, pp. 2203-7, (1996) (PubMed).

Dempsey, Allison, Akkaraju, Goodnow, Fearon: "C3d of complement as a molecular adjuvant: bridging innate and acquired immunity." in: Science (New York, N.Y.), Vol. 271, Issue 5247, pp. 348-50, (1996) (PubMed).

Molina, Holers, Li, Fung, Mariathasan, Goellner, Strauss-Schoenberger, Karr, Chaplin: "Markedly impaired humoral immune response in mice deficient in complement receptors 1 and 2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 8, pp. 3357-61, (1996) (PubMed).

Tedder, Zhou, Engel: "The CD19/CD21 signal transduction complex of B lymphocytes." in: Immunology today, Vol. 15, Issue 9, pp. 437-42, (1994) (PubMed).

Martin, Weis: "Murine macrophages lack expression of the Cr2-145 (CR2) and Cr2-190 (CR1) gene products." in: European journal of immunology, Vol. 23, Issue 11, pp. 3037-42, (1993) (PubMed).

Yoshida, van den Berg, Dijkstra: "Two functionally different follicular dendritic cells in secondary lymphoid follicles of mouse spleen, as revealed by CR1/2 and FcR gamma II-mediated immune-complex trapping." in: Immunology, Vol. 80, Issue 1, pp. 34-9, (1993) (PubMed).

Kinoshita, Thyphronitis, Tsokos, Finkelman, Hong, Sakai, Inoue: "Characterization of murine complement receptor type 2 and its immunological cross-reactivity with type 1 receptor." in: International immunology, Vol. 2, Issue 7, pp. 651-9, (1991) (PubMed).

Thyphronitis, Kinoshita, Inoue, Schweinle, Tsokos, Metcalf, Finkelman, Balow: "Modulation of mouse complement receptors 1 and 2 suppresses antibody responses in vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 1, pp. 224-30, (1991) (PubMed).

Wiersma, Kinoshita, Heyman: "Inhibition of immunological memory and T-independent humoral responses by monoclonal antibodies specific for murine complement receptors." in: European journal of immunology, Vol. 21, Issue 10, pp. 2501-6, (1991) (PubMed).

Heyman, Wiersma, Kinoshita: "In vivo inhibition of the antibody response by a complement receptor-specific monoclonal antibody." in: The Journal of experimental medicine, Vol. 172, Issue 2, pp. 665-8, (1990) (PubMed).

Kurtz, OToole, Christensen, Weis: "The murine complement receptor gene family. IV. Alternative splicing of Cr2 gene transcripts predicts two distinct gene products that share homologous domains with both human CR2 and CR1." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 9, pp. 3581-91, (1990) (PubMed).

Kinoshita, Takeda, Hong, Kozono, Sakai, Inoue: "Monoclonal antibodies to mouse complement receptor type 1 (CR1). Their use in a distribution study showing that mouse erythrocytes and platelets are CR1-negative." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 140, Issue 9, pp. 3066-72, (1988) (PubMed).

Target Details for CD21

Target: CD21 (CR2) (Complement Component (3d/Epstein Barr Virus) Receptor 2 (CR2))

Alternative Name: CD21/CD35 (CR2 Products)

Synonyms: C3DR antibody, CD21 antibody, CR antibody, CVID7 antibody, SLEB9 antibody, Cd21 antibody, cd21 antibody, CR1 antibody, CR1-L antibody, CR2 antibody, complement C3d receptor 2 antibody, complement C3b/C4b receptor 1 (Knops blood group) antibody, CR2 antibody, Cr2 antibody, CR1 antibody

Background: Synonyms: CR2/CR1

Pathways: Complement System