CD24 antibody (Biotin)

Catalog No. ABIN2689110

Product Details anti-CD24 Antibody (Biotin)

Target: CD24 (CD24 Molecule (CD24))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD24 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Characteristics: The M1/69 antibody reacts with CD24 (Heat-Stable Antigen, HSA or HsAg), a variably glycosylated GPI-anchored membrane protein found on erythrocytes, granulocytes, monocytes, lymphocytes, and neurons. Hematopoietic stem cells of the embryonic yolk sac and fetal liver express CD24.5 Levels of expression of CD24 vary during differentiation of the T and B cell lineages. In the bone marrow, hematopoietic progenitors acquire CD24 expression upon commitment to the B-lymphocyte lineage. Immature B cells in the bone marrow and spleen of adult mice peripheral B lymphocytes express intermediate levels of CD24. The level of CD24 expression has been reported to rise upon activation of splenic B cells with LPS, but not with CD154 (CD40 Ligand). The majority of thymocytes express high levels of CD24, while most mature thymic and peripheral T lymphocytes do not express CD24. In contrast, TCR-bearing thymocytes which emigrate to the spleen are CD24+. Dendritic cells of the thymus, spleen, liver, and epidermal Langerhans cells have also been reported to express CD24. CD24 is not expressed by NK cells, as determined by staining with J11d mAb (Cat. No. 553146). CD24 is involved in the costimulation of CD4+ T cells by B cells, it is a "co-inducer" of in vitro thymocyte maturation, and it is a ligand of CD62P (P-selectin). While the monoclonal antibodies 30-F1, M1/69, and J11d all react with CD24, they show subtle differences in the level of staining of different lymphocyte populations. When possible, investigators should continue to use the same monoclonal antibody as used in previous studies. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Differential expression of CD24 on thymocytes and peripheral T lymphocytes. C57BL/6 thymocytes were stained with biotin-conjugated mAb M1/69 followed by Avidin-FITC (Cat. No. 554057, left panel). C57BL/6 splenocytes were simultaneously stained with biotin-conjugated mAb M1/69 and PE-conjugated anti-mouse CD3e mAb 145-2C11 (Cat. No. 553063/553064) followed by Avidin-FITC (right panel). Flow cytometry was performed on a BD FACScan™ flow cytometry system. Page 1 of 2553260 Rev. 1

BD Pharmingen™ Biotin Rat Anti-Mouse CD24 - Biotin - Clone M1/69 - Isotype Rat IgG2b, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: C57BL/10 Mouse Splenic T Lymphocytes

Clone: M1-69

Isotype: IgG2b kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-CD24 antibody (Biotin) (ABIN2689110)

Hunte, Capone, Zlotnik, Rennick, Moore: "Acquisition of CD24 expression by Lin-CD43+B220(low)ckit(hi) cells coincides with commitment to the B cell lineage." in: European journal of immunology, Vol. 28, Issue 11, pp. 3850-6, (1998) (PubMed).

Calaora, Chazal, Nielsen, Rougon, Moreau: "mCD24 expression in the developing mouse brain and in zones of secondary neurogenesis in the adult." in: Neuroscience, Vol. 73, Issue 2, pp. 581-94, (1997) (PubMed).

Cibotti, Punt, Dash, Sharrow, Singer: "Surface molecules that drive T cell development in vitro in the absence of thymic epithelium and in the absence of lineage-specific signals." in: Immunity, Vol. 6, Issue 3, pp. 245-55, (1997) (PubMed).

Aigner, Ruppert, Hubbe, Sammar, Sthoeger, Butcher, Vestweber, Altevogt: "Heat stable antigen (mouse CD24) supports myeloid cell binding to endothelial and platelet P-selectin." in: International immunology, Vol. 7, Issue 10, pp. 1557-65, (1996) (PubMed).

Auerbach, Huang, Lu: "Hematopoietic stem cells in the mouse embryonic yolk sac." in: Stem cells (Dayton, Ohio), Vol. 14, Issue 3, pp. 269-80, (1996) (PubMed).

Kennedy, Mohler, Shanebeck, Baum, Picha, Otten-Evans, Janeway, Grabstein: "Induction of B cell costimulatory function by recombinant murine CD40 ligand." in: European journal of immunology, Vol. 24, Issue 1, pp. 116-23, (1994) (PubMed).

Wenger, Rochelle, Seldin, Köhler, Nielsen: "The heat stable antigen (mouse CD24) gene is differentially regulated but has a housekeeping promoter." in: The Journal of biological chemistry, Vol. 268, Issue 31, pp. 23345-52, (1993) (PubMed).

Vremec, Zorbas, Scollay, Saunders, Ardavin, Wu, Shortman: "The surface phenotype of dendritic cells purified from mouse thymus and spleen: investigation of the CD8 expression by a subpopulation of dendritic cells." in: The Journal of experimental medicine, Vol. 176, Issue 1, pp. 47-58, (1992) (PubMed).

Alterman, Crispe, Kinnon: "Characterization of the murine heat-stable antigen: an hematolymphoid differentiation antigen defined by the J11d, M1/69 and B2A2 antibodies." in: European journal of immunology, Vol. 20, Issue 7, pp. 1597-602, (1990) (PubMed).

Wilson, Day, Scollay, Shortman: "Subpopulations of mature murine thymocytes: properties of CD4-CD8+ and CD4+CD8- thymocytes lacking the heat-stable antigen." in: Cellular immunology, Vol. 117, Issue 2, pp. 312-26, (1989) (PubMed).

Crowley, Inaba, Witmer-Pack, Steinman: "The cell surface of mouse dendritic cells: FACS analyses of dendritic cells from different tissues including thymus." in: Cellular immunology, Vol. 118, Issue 1, pp. 108-25, (1989) (PubMed).

Target Details for CD24

Target: CD24 (CD24 Molecule (CD24))

Alternative Name: CD24 (CD24 Products)

Synonyms: CD24A antibody, Cd24a antibody, CD24 molecule antibody, CD24 antibody, Cd24 antibody

Background: Synonyms: Heat Stable Antigen

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation