TRAIL antibody (Biotin)

Catalog No. ABIN2689130

Product Details anti-TRAIL Antibody (Biotin)

Target: TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TRAIL antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Characteristics: TRAIL (TNF-Related Apoptosis-Inducing Ligand), also known as Apo2L, is a member of the TNF ligand family. TRAIl is a type II membrane protein which may be expressed as a full-length, cell surface associated protein as well as in a soluble form. Both surface and soluble forms of TRAIL rapidly induce apoptosis on a wide range of cell lines. TRAIL has been shown to cause apoptotic death in either tumorigenic or transformed cells, but not in normal cells. TRAIL-mediated apoptosis has been shown to involve the activation of caspases, and is blocked by over-expression of the caspase-1 protease inhibitor, CrmA. TRAIL has also been reported to induce the transcription factor NF-κB in a cell type-specific manner. Two cognate TRAIL receptors DR4, and DR5, as well as two decoy receptors, DcR1/TRID and DcR2/TRUNDD have been identified. TRAIL has been shown to be involved in T cell cytotoxicity, but the exact physiological role TRAIL plays in T-cell mediated cytotoxicity remains to be elucidated. The RIK-2 antibody recognizes human TRAIL. Human TRAIL cDNA was transferred to an expression vector and transfected into the 2PK-3 mouse B cell lymphoma cell line to generate stable transfectants, which were then used to immunize mice. The RIK-2 clone was selected based on its ability to block cytotoxic activity. TRAIL has been renamed as CD253 recently. Flow cytometric analysis of TRAIL. Profile of human TRAIL/2PK-3 cell line analyzed on a FACScan™

BD Pharmingen™ Biotin Mouse Anti-Human CD253 - Biotin - Clone RIK-2 - Isotype Mouse IgG1 - Reactivity Hu - 0.2 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human TRAIL

Clone: RIK

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry : Applications include flow cytometry (0.25-1.0 μg/1x10e6 cells). Blocking: The RIK-2 antibody is useful to block TRAIL-induced apoptosis. The no azide/low endotoxin format (NA/LE), Cat. No. 550912, is recommended for in vitro blocking assays.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-TRAIL antibody (Biotin) (ABIN2689130)

Kayagaki, Yamaguchi, Nakayama, Kawasaki, Akiba, Okumura, Yagita: "Involvement of TNF-related apoptosis-inducing ligand in human CD4+ T cell-mediated cytotoxicity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 5, pp. 2639-47, (1999) (PubMed).

Mariani, Matiba, Armandola, Krammer: "Interleukin 1 beta-converting enzyme related proteases/caspases are involved in TRAIL-induced apoptosis of myeloma and leukemia cells." in: The Journal of cell biology, Vol. 137, Issue 1, pp. 221-9, (1997) (PubMed).

Sheridan, Marsters, Pitti, Gurney, Skubatch, Baldwin, Ramakrishnan, Gray, Baker, Wood, Goddard, Godowski, Ashkenazi: "Control of TRAIL-induced apoptosis by a family of signaling and decoy receptors." in: Science (New York, N.Y.), Vol. 277, Issue 5327, pp. 818-21, (1997) (PubMed).

Pitti, Marsters, Ruppert, Donahue, Moore, Ashkenazi: "Induction of apoptosis by Apo-2 ligand, a new member of the tumor necrosis factor cytokine family." in: The Journal of biological chemistry, Vol. 271, Issue 22, pp. 12687-90, (1996) (PubMed).

Wiley, Schooley, Smolak, Din, Huang, Nicholl, Sutherland, Smith, Rauch, Smith: "Identification and characterization of a new member of the TNF family that induces apoptosis." in: Immunity, Vol. 3, Issue 6, pp. 673-82, (1996) (PubMed).

Target Details for TRAIL

Target: TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))

Alternative Name: CD253 (TNFSF10 Products)

Synonyms: APO2L antibody, Apo-2L antibody, CD253 antibody, TL2 antibody, TRAIL antibody, A330042I21Rik antibody, AI448571 antibody, APO-2L antibody, Ly81 antibody, Trail antibody, tl2 antibody, apo2l antibody, cd253 antibody, trail antibody, apo-2l antibody, trail1 antibody, xtrail1 antibody, tnfsf10l2 antibody, zgc:92320 antibody, TNLG6A antibody, TNF superfamily member 10 antibody, tumor necrosis factor (ligand) superfamily, member 10 antibody, tumor necrosis factor superfamily member 10 antibody, TNF superfamily member 10 S homeolog antibody, TNFSF10 antibody, Tnfsf10 antibody, tnfsf10.S antibody, tnfsf10 antibody

Background: Synonyms: TRAIL, APO-2L, TL2

Pathways: Apoptosis, Positive Regulation of Endopeptidase Activity