Prostaglandin D2 Receptor 2 (PTGDR2) antibody

Catalog No. ABIN2689160

Product Details

Target: Prostaglandin D2 Receptor 2 (PTGDR2)

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Characteristics: Monoclonal antibody BM16 reacts with CRTH2 (chemoattractant receptor-homologous molecule expressed on Th2 cells). CRTH2 is a member of the G protein-coupled leukocyte chemoattractant receptor family and it is expressed on Th2, but not Th1 lineage cells. It is detectable on CD4+ T cells in fresh PBMC and no significant expression on B cells and NK cells. CRTH2 is also expressed on peripheral blood basophils and eosinophils, suggesting its involvement allergic reactions. Phenotypic analysis of CD4+ T cells expressing CRTH2 demonstrated CD45RA-, CD45RO+ and CD25+. These cells produce Th2- but little or no Th1-type cytokines upon stimulation with PMA and Ionomycin. Profile of anti-CRTH2 (BM16) reactivity on HUT 78 cells analyzed by flow cytometry. Second step staining with Cat. No. 554016.

BD Pharmingen™ Purified Rat Anti-Human CD294 - Purified - Clone BM16 - Isotype Rat IgG2a, κ - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Transfectant cells

Clone: BM16

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References

Cosmi, Annunziato, Galli MIG, Maggi RME, Nagata, Romagnani: "CRTH2 is the most reliable marker for the detection of circulating human type 2 Th and type 2 T cytotoxic cells in health and disease." in: European journal of immunology, Vol. 30, Issue 10, pp. 2972-9, (2000) (PubMed).

Nagata, Hirai, Tanaka, Ogawa, Aso, Sugamura, Nakamura, Takano: "CRTH2, an orphan receptor of T-helper-2-cells, is expressed on basophils and eosinophils and responds to mast cell-derived factor(s)." in: FEBS letters, Vol. 459, Issue 2, pp. 195-9, (1999) (PubMed).

Nagata, Tanaka, Ogawa, Kemmotsu, Imai, Yoshie, Abe, Tada, Nakamura, Sugamura, Takano: "Selective expression of a novel surface molecule by human Th2 cells in vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 3, pp. 1278-86, (1999) (PubMed).

Target Details

Target: Prostaglandin D2 Receptor 2 (PTGDR2)

Alternative Name: CD294 (PTGDR2 Products)

Synonyms: CD294 antibody, CRTH2 antibody, DL1R antibody, DP2 antibody, GPR44 antibody, Crth2 antibody, Grp45 antibody, Ptgdr2 antibody, Gpr44 antibody, prostaglandin D2 receptor 2 antibody, PTGDR2 antibody, Ptgdr2 antibody

Background: Synonyms: CRTH2

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling