CD45RA antibody (Biotin)

Catalog No. ABIN2689288

Product Details anti-CD45RA Antibody (Biotin)

Target: CD45RA

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD45RA antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Characteristics: Reacts with the 220 kDa isoform of the human leukocyte common antigen found on approximately 40-50 % of peripheral CD4+ T cells, 50 % of peripheral CD8+ T cells and on a portion of B cells and monocytes. T cells expressing this antigen are naive or virgin T cells. CD45RA antibodies are useful for the study of the suppressor/inducer subpopulation of CD4+ lymphocytes. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Profile of peripheral blood lymphocytes analyzed on a FACScan

BD Pharmingen™ Biotin Mouse Anti-Human CD45RA - Biotin - Clone HI100 - Isotype Mouse IgG2b, κ - Reactivity Hu - 100 Tests

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Clone: HI100

Isotype: IgG2b kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 20 μL

Restrictions: For Research Use only

Handling

Buffer: Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-CD45RA antibody (Biotin) (ABIN2689288)

Guesdon, Ternynck, Avrameas: "The use of avidin-biotin interaction in immunoenzymatic techniques." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 27, Issue 8, pp. 1131-9, (1979) (PubMed).

Target Details for CD45RA

Target: CD45RA

Alternative Name: CD45RA (CD45RA Products)

Synonyms: B220 antibody, CD45 antibody, CD45R antibody, GP180 antibody, L-CA antibody, LCA antibody, LY5 antibody, T200 antibody, Lca antibody, RT7 antibody, protein tyrosine phosphatase, receptor type C antibody, protein tyrosine phosphatase, receptor type, C antibody, PTPRC antibody, Ptprc antibody