Integrin beta 3 antibody

Catalog No. ABIN2689353

Product Details anti-Integrin beta 3 Antibody

Target: Integrin beta 3 (ITGB3)

Reactivity: Mouse, Rat

Host: Armenian Hamster

Clonality: Monoclonal

Conjugate: This Integrin beta 3 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: BD Pharmingen™

Characteristics: The 2C9.G2 antibody reacts with the integrin β3 chain (CD61), which associates with the integrin αv chain (CD51) to form the vitronectin receptor and with the αIIb chain (CD41) to form the gpIIb/IIIa complex. Both receptors mediate adhesion to fibronectin, fibrinogen, vitronectin, thrombospondin, and von Willebrand factor. Leukocyte-endothelial adhesion is also mediated by the binding of αvβ3 integrin or vitronectin receptor to CD31 (PECAM-1). In addition, interaction of the αvβ3 integrin with its ligands regulates the L-type Ca2+ channel in vascular smooth muscle cells, possibly mediating vasodilatory responses to injury. Soluble and insoluble 2C9.G2 mAb mimics the effect of the natural ligands in smooth muscle cells from rat cremaster arterioles. Furthermore, osteopontin, also named Eta-1, is a cytokine that binds to αvβ3. CD61 is expressed on platelets, activated T lymphocytes, polymorphonuclear granulocytes, and blastocysts. Cross-reactivity of mAb 2C9.G2 to rat mast cells and platelets has been observed by flow cytometric analysis. mAb 2C9.G2 has been demonstrated to block binding of rat and mouse cells to fibronectin. Immunohistochemical staining of CD61 positive cells. Frozen sections of normal mouse spleen were reacted with the anti-CD61 antibody. Cells positive for CD61 can be identified by the brown labeling of their cell membranes. Amplification 20X.

BD Pharmingen™ Purified Hamster Anti-Mouse CD61 - Purified - Clone 2C9.G2 - Isotype Armenian Hamster IgG1, κ - Reactivity Ms, Rat - 1 mL

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse T-cell Hybridoma 2B4 Vitronectin Receptor

Clone: 2C9-G2

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-Integrin beta 3 antibody (ABIN2689353)

Ashkar, Weber, Panoutsakopoulou, Sanchirico, Jansson, Zawaideh, Rittling, Denhardt, Glimcher, Cantor: "Eta-1 (osteopontin): an early component of type-1 (cell-mediated) immunity." in: Science (New York, N.Y.), Vol. 287, Issue 5454, pp. 860-4, (2000) (PubMed).

Piali, Hammel, Uherek, Bachmann, Gisler, Dunon, Imhof: "CD31/PECAM-1 is a ligand for alpha v beta 3 integrin involved in adhesion of leukocytes to endothelium." in: The Journal of cell biology, Vol. 130, Issue 2, pp. 451-60, (1995) (PubMed).

Yasuda, Hasunuma, Adachi, Sekine, Sakanishi, Hashimoto, Ra, Yagita, Okumura: "Expression and function of fibronectin binding integrins on rat mast cells." in: International immunology, Vol. 7, Issue 2, pp. 251-8, (1995) (PubMed).

Kieffer, Phillips: "Platelet membrane glycoproteins: functions in cellular interactions." in: Annual review of cell biology, Vol. 6, pp. 329-57, (1991) (PubMed).

Moulder, Roberts, Shevach, Coligan: "The mouse vitronectin receptor is a T cell activation antigen." in: The Journal of experimental medicine, Vol. 173, Issue 2, pp. 343-7, (1991) (PubMed).

Target Details for Integrin beta 3

Target: Integrin beta 3 (ITGB3)

Alternative Name: CD61 (ITGB3 Products)

Synonyms: BDPLT16 antibody, BDPLT2 antibody, CD61 antibody, GP3A antibody, GPIIIa antibody, GT antibody, INGRB3 antibody, integrin antibody, INTB3 antibody, GPIIIA antibody, cd61 antibody, gp3a antibody, itgb3 antibody, itgb3.2 antibody, itgb3.1 antibody, integrin subunit beta 3 antibody, integrin beta 3 antibody, integrin subunit beta 3 L homeolog antibody, integrin beta 3a antibody, integrin beta 3b antibody, ITGB3 antibody, Itgb3 antibody, itgb3.L antibody, itgb3a antibody, itgb3b antibody

Background: Synonyms: Integrin β3 chain

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Integrin Complex