FCGR1A antibody (Biotin)

Catalog No. ABIN2689372

Product Details anti-FCGR1A Antibody (Biotin)

Target: FCGR1A (Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FCGR1A antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Characteristics: The monoclonal antibody 10.1 reacts with CD64, a 75 kD type I transmembrane glycoprotein that is a high affinity receptor for IgG (FcγRI). CD64, a key receptor in the development of immune responses, has a dual role as a low affinity receptor for IgG3 and high affinity receptor for IgG2a linking innate and adaptive immunities. CD64 mediates endocytosis, phagocytosis, antibody-dependent cellular toxicity, cytokine release and superoxide generation. CD64 is expressed largely on macrophages and dendritic cells. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Profile of peripheral blood monocytes analyzed on a FACScan

BD Pharmingen™ Biotin Mouse Anti-Human CD64 - Biotin - Clone 10.1 - Isotype Mouse IgG1, κ - Reactivity Hu - 100 Tests

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Clone: 10-1

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 20 μL

Restrictions: For Research Use only

Handling

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-FCGR1A antibody (Biotin) (ABIN2689372)

Indik, Hunter, Huang, Pan, Chien, Kelly, Levinson, Kimberly, Schreiber: "The high affinity Fc gamma receptor (CD64) induces phagocytosis in the absence of its cytoplasmic domain: the gamma subunit of Fc gamma RIIIA imparts phagocytic function to Fc gamma RI." in: Experimental hematology, Vol. 22, Issue 7, pp. 599-606, (1994) (PubMed).

Dougherty, Selvendran, Murdoch, Palmer, Hogg: "The human mononuclear phagocyte high-affinity Fc receptor, FcRI, defined by a monoclonal antibody, 10.1." in: European journal of immunology, Vol. 17, Issue 10, pp. 1453-9, (1987) (PubMed).

Guesdon, Ternynck, Avrameas: "The use of avidin-biotin interaction in immunoenzymatic techniques." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 27, Issue 8, pp. 1131-9, (1979) (PubMed).

Target Details for FCGR1A

Target: FCGR1A (Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A))

Alternative Name: CD64 (FCGR1A Products)

Synonyms: CD64 antibody, CD64A antibody, FCRI antibody, IGFR1 antibody, FcgammaRI antibody, Fcgr1 antibody, Fcgr1b antibody, AI323638 antibody, AV092959 antibody, IGGHAFC antibody, FCGR1A antibody, Fc fragment of IgG receptor Ia antibody, Fc receptor, IgG, high affinity I antibody, FCGR1A antibody, Fcgr1a antibody, Fcgr1 antibody

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process