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CD86 antibody

CD86 Reactivity: Rat FACS, IP, IHC (fro) Host: Mouse Monoclonal 24F unconjugated
Catalog No. ABIN2689421
  • Target See all CD86 Antibodies
    CD86
    Reactivity
    • 139
    • 116
    • 50
    • 20
    • 10
    • 7
    • 5
    • 4
    • 3
    • 3
    • 2
    Rat
    Host
    • 79
    • 69
    • 67
    Mouse
    Clonality
    • 152
    • 60
    • 1
    Monoclonal
    Conjugate
    • 86
    • 23
    • 18
    • 14
    • 13
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD86 antibody is un-conjugated
    Application
    • 145
    • 97
    • 44
    • 41
    • 33
    • 31
    • 30
    • 23
    • 16
    • 16
    • 15
    • 14
    • 13
    • 11
    • 5
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Brand
    BD Pharmingen™
    Characteristics
    The 24F antibody reacts with CD86 (B7-2), a member of the Ig superfamily of transmembrane proteins. CD86, a ligand for CD28 and CD152 (CTLA-4), is one of the accessory molecules that plays an important role in T cell-B cell costimulatory interactions. CD86 is predominantly expressed on antigen-presenting cells. It can be upregulated on splenic B cells by in vitro polyclonal activation, such as LPS treatment. 24F mAb is reported to block the costimulatory function of rat CD86 and to immunoprecipitate CD86 from Lewis-S1 cell lysates. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

    BD Pharmingen™ Purified Mouse Anti-Rat CD86 - Purified - Clone 24F - Isotype Mouse IgG1, κ - Reactivity Rat - 0.5 mg
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    HTLV-1-transformed rat T-cell line Lewis-S1
    Clone
    24F
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product CD86 Primary Antibody
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Store undiluted at 4°C.
  • Damoiseaux, Yagita, Okumura, van Breda Vriesman: "Costimulatory molecules CD80 and CD86 in the rat; tissue distribution and expression by antigen-presenting cells." in: Journal of leukocyte biology, Vol. 64, Issue 6, pp. 803-9, (1998) (PubMed).

    Maeda, Sato, Azuma, Yagita, Okumura: "Characterization of rat CD80 and CD86 by molecular cloning and mAb." in: International immunology, Vol. 9, Issue 7, pp. 993-1000, (1997) (PubMed).

    Bluestone: "New perspectives of CD28-B7-mediated T cell costimulation." in: Immunity, Vol. 2, Issue 6, pp. 555-9, (1995) (PubMed).

  • Target
    CD86
    Alternative Name
    CD86 (CD86 Products)
    Synonyms
    B7-2 antibody, B7.2 antibody, B70 antibody, CD28LG2 antibody, LAB72 antibody, B7 antibody, CLS1 antibody, Cd28l2 antibody, ETC-1 antibody, Ly-58 antibody, Ly58 antibody, MB7 antibody, MB7-2 antibody, TS/A-2 antibody, CD86 molecule antibody, CD86 antigen antibody, integrin subunit alpha 2b antibody, CD86 antibody, Cd86 antibody, Itga2b antibody
    Background
    Synonyms: B7-2
    Pathways
    TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
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