CD94 antibody (Biotin)

Catalog No. ABIN2689459

Product Details anti-CD94 Antibody (Biotin)

Target: CD94 (KLRD1) (Killer Cell Lectin-Like Receptor Subfamily D, Member 1 (KLRD1))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD94 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Brand: BD Pharmingen™

Characteristics: The 18d3 antibody reacts with CD94 on all NK cells, NK1.1- or DX5-positive T lymphocytes (NK-T cells), and a subset of CD8-positive T lymphocytes in most strains tested (eg, A/J, AKR/J, BALB/c, C3H/He, C57BL/6, CBA/J, DBA/1, FVB/N, 129/Sv, NOD, SWR, and most DBA/2 substrains, but not DBA/2J). DBA/2J mice do not express CD94.3 CD94 is also expressed on CD8+ T lymphocytes activated in vivo. CD94 is a type-II transmembrane protein with an extracellular lectin-like domain and a short cytoplasmic tail which is not believed to have any signalling function. Heterodimers of CD94 with NKG2A, NKG2C, or NKG2E recognize Qa-1 (a non-classical MHC class I antigen) presenting the Qdm peptide. Studies on CD94/NKG2 heterodimers on human NK cells have demonstrated that the NKG2 components mediate signal transduction for the receptor, NKG2A being inhibitory and NKG2C being stimulatory. Similarly, the mouse NKG2A molecule contains two intracytoplasmic sequences which resemble the ITIM (Immunoreceptor Tyrosine-based Inhibitory Motif) consensus sequence. CD94/NKG2 receptors appear on fetal NK cells before the Ly- 49 MHC class I receptors, suggesting that CD94/NKG2 receptors and their ligand, Qa-1, may play a role in maintenance of self-tolerance in developing NK cells. Expression of CD94 on mouse splenic NK cells. C57BL/6 splenocytes were preincubated with Mouse BD Fc Block™ purified anti-mouse CD16/CD32 mAb 2.4G2 (Cat. no. 553141/553142), then simultaneously stained with biotinconjugated rat IgG2a isotype control mAb R35-95 (Cat. no. 553928, Left panel) or biotinylated mAb 18d3 (Right panel) and APC-conjugated anti- mouse NK-1.1 mAb PK136 (Cat. no. 550627), followed by Streptavidin-PE (Cat. no. 554061). The CD94[dim] and CD94[bright] subpopulations of NK cells are observed in all strains tested, although the relative proportions of the two subsets may vary. Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.

BD Pharmingen™ Biotin Rat Anti-Mouse CD94 - Biotin - Clone 18d3 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Transfected cell line

Clone: 18d3

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-CD94 antibody (Biotin) (ABIN2689459)

McMahon, Zajac, Jamieson, Corral, Hammer, Ahmed, Raulet: "Viral and bacterial infections induce expression of multiple NK cell receptors in responding CD8(+) T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 3, pp. 1444-52, (2002) (PubMed).

Vance, Jamieson, Cado, Raulet: "Implications of CD94 deficiency and monoallelic NKG2A expression for natural killer cell development and repertoire formation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 2, pp. 868-73, (2002) (PubMed).

Vance, Jamieson, Raulet: "Recognition of the class Ib molecule Qa-1(b) by putative activating receptors CD94/NKG2C and CD94/NKG2E on mouse natural killer cells." in: The Journal of experimental medicine, Vol. 190, Issue 12, pp. 1801-12, (2000) (PubMed).

Toomey, Salcedo, Cotterill, Millrain, Chrzanowska-Lightowlers, Lawry, Fraser, Gays, Robinson, Shrestha, Dyson, Brooks et al.: "Stochastic acquisition of Qa1 receptors during the development of fetal NK cells in vitro accounts in part but not in whole for the ability of these cells to distinguish between class I-sufficient ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 6, pp. 3176-84, (1999) (PubMed).

Vance, Kraft, Altman, Jensen, Raulet: "Mouse CD94/NKG2A is a natural killer cell receptor for the nonclassical major histocompatibility complex (MHC) class I molecule Qa-1(b)." in: The Journal of experimental medicine, Vol. 188, Issue 10, pp. 1841-8, (1999) (PubMed).

Ho, Heusel, Brown, Matsumoto, Scalzo, Yokoyama: "Murine Nkg2d and Cd94 are clustered within the natural killer complex and are expressed independently in natural killer cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 11, pp. 6320-5, (1998) (PubMed).

Yokoyama: "Natural killer cell receptors." in: Current opinion in immunology, Vol. 10, Issue 3, pp. 298-305, (1998) (PubMed).

Target Details for CD94

Target: CD94 (KLRD1) (Killer Cell Lectin-Like Receptor Subfamily D, Member 1 (KLRD1))

Alternative Name: CD94 (KLRD1 Products)

Synonyms: CD94 antibody, CD94-B antibody, Cd94 antibody, killer cell lectin like receptor D1 antibody, killer cell lectin-like receptor subfamily D, member 1 antibody, killer cell lectin-like receptor, subfamily D, member 1 antibody, KLRD1 antibody, Klrd1 antibody