Cyclin E1 antibody
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- Target See all Cyclin E1 (CCNE1) Antibodies
- Cyclin E1 (CCNE1)
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Cyclin E1 antibody is un-conjugated
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Application
- Immunoprecipitation (IP)
- Brand
- BD Pharmingen™
- Characteristics
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Cyclins and cyclin-dependent kinases (cdks) are evolutionarily conserved proteins that are essential for cell-cycle control in eukaryotes. Cyclins (regulatory subunits) bind to cdks (catalytic subunits) to form complexes that regulate the progression of the cell cycle. The main cyclin-cdks complexes formed in vertebrate cells are cyclin D-cdk4 (G0/G1), cyclin E-cdk2 (G1/S), cyclin A-cdk2 (S) and cyclin B1-cdk1 (G2/M). These complexes are regulated by activating and inhibitory phosphorylation events, as well as by interactions with small proteins that bind to cyclins, cdks, or cyclin-cdk complexes, e.g., p21 and p27[Kip1]. Specific substrates for cdk-cyclin complexes include nuclear lamins, histones, oncogenes (c-src, c-abl, SV40 large T-Ag), tumor suppressor genes (e.g., retinoblastoma protein [Rb] and p53), nucleolin, RNA polymerase II and others. Cyclin E is expressed in G1 and associates with cdk2 to form an active kinase. Cyclin E-cdk2 complexes play an important role in regulation of the G1/S restriction checkpoint in the cell cycle. Aberrant expression of cyclin E is associated with oncogenic transformation of cells. Cyclin E migrates at a reduced molecular weight of ~50 kDa.
BD Pharmingen™ Purified Mouse Anti-Human Cyclin E - Purified - Clone HE67 - Isotype Mouse IgG2b - Reactivity Hu - 0.1 mg - Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogen
- Recombinant human Cyclin E
- Clone
- HE67
- Isotype
- IgG2b
- Top Product
- Discover our top product CCNE1 Primary Antibody
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Store undiluted at 4°C.
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The role of cyclin E in cell proliferation, development and cancer." in: Progress in cell cycle research, Vol. 3, pp. 171-91, (1998) (PubMed).
: "Cyclin E, a potential prognostic marker for breast cancer." in: Cancer research, Vol. 54, Issue 2, pp. 380-5, (1994) (PubMed).
: "The adenovirus E1A-associated kinase consists of cyclin E-p33cdk2 and cyclin A-p33cdk2." in: Journal of virology, Vol. 67, Issue 5, pp. 2456-65, (1993) (PubMed).
: "Mammalian G1 cyclins." in: Cell, Vol. 73, Issue 6, pp. 1059-65, (1993) (PubMed).
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The role of cyclin E in cell proliferation, development and cancer." in: Progress in cell cycle research, Vol. 3, pp. 171-91, (1998) (PubMed).
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- Target
- Cyclin E1 (CCNE1)
- Alternative Name
- Cyclin E (CCNE1 Products)
- Synonyms
- CCNE1 antibody, ccne antibody, cyclinE antibody, CCNE antibody, AW538188 antibody, CycE1 antibody, CYCLE antibody, Ccne antibody, cyce2 antibody, cycE3 antibody, cyce antibody, fa18e07 antibody, wu:fa18e07 antibody, cyclin E1 antibody, cyclin E1 L homeolog antibody, cyclin E1 S homeolog antibody, CCNE1 antibody, ccne1 antibody, Ccne1 antibody, ccne1.L antibody, ccne1.S antibody
- Molecular Weight
- 50 kDa
- Pathways
- Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases
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