VEGFR2/CD309 antibody

Catalog No. ABIN2689505

Product Details anti-VEGFR2/CD309 Antibody

Target: VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This VEGFR2/CD309 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Brand: BD Pharmingen™

Characteristics: The Avas 12α1 monoclonal antibody specifically binds to fetal liver kinase 1 (Flk-1), a receptor protein tyrosine kinase closely related to CD117 (c-kit) and CD140a (PDGF Receptor α chain) of the immunoglobulin superfamily. Flk-1, also known as VEGF Receptor-2 (VEGF-R2), is a receptor for vascular endothelial growth factor (VEGF). It is expressed, at the mRNA and protein levels, on distinct sets of mesoderm during gastrulation and on endothelial cells in embryonic and adult tissues. In vivo and in vitro studies indicate that Flk-1 is required for the embryonic development of vascular endothelial and hematopoietic cells. Immunohistochemical staining of mouse Flk-1. Frozen sections of normal mouse spleen were reacted with the anti-Flk-1 antibody. Endothelial cells expressing Flk-1 can be identified by the brown labeling of their cell membranes. Amplification 20X.

BD Pharmingen™ Purified Rat Anti-Mouse Flk-1 - Purified - Clone Avas 12alpha1 - Isotype Rat IgG2a, κ - Reactivity Ms - 1 mL

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse Flk-1 Recombinant Protein

Clone: Avas 12alpha1

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 31.25 μg/mL

Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-VEGFR2/CD309 antibody (ABIN2689505)

Nishikawa, Nishikawa, Kawamoto, Yoshida, Kizumoto, Kataoka, Katsura: "In vitro generation of lymphohematopoietic cells from endothelial cells purified from murine embryos." in: Immunity, Vol. 8, Issue 6, pp. 761-9, (1998) (PubMed).

Hanahan: "Signaling vascular morphogenesis and maintenance." in: Science (New York, N.Y.), Vol. 277, Issue 5322, pp. 48-50, (1997) (PubMed).

Shalaby, Ho, Stanford, Fischer, Schuh, Schwartz, Bernstein, Rossant: "A requirement for Flk1 in primitive and definitive hematopoiesis and vasculogenesis." in: Cell, Vol. 89, Issue 6, pp. 981-90, (1997) (PubMed).

Shalaby, Rossant, Yamaguchi, Gertsenstein, Wu, Breitman, Schuh: "Failure of blood-island formation and vasculogenesis in Flk-1-deficient mice." in: Nature, Vol. 376, Issue 6535, pp. 62-6, (1995) (PubMed).

Millauer, Wizigmann-Voos, Schnürch, Martinez, Møller, Risau, Ullrich: "High affinity VEGF binding and developmental expression suggest Flk-1 as a major regulator of vasculogenesis and angiogenesis." in: Cell, Vol. 72, Issue 6, pp. 835-46, (1993) (PubMed).

Target Details for VEGFR2/CD309

Target: VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))

Alternative Name: Flk-1 (VEGFR2 Products)

Synonyms: CD309 antibody, FLK1 antibody, VEGFR antibody, VEGFR2 antibody, 6130401C07 antibody, Flk-1 antibody, Flk1 antibody, Krd-1 antibody, Ly73 antibody, VEGFR-2 antibody, sVEGFR-2 antibody, Vegfr-2 antibody, FLK-1 antibody, flk-1 antibody, vegfr-2 antibody, KDR antibody, FLK/KDR antibody, flk1 antibody, flk1b antibody, kdrb antibody, si:busm1-205d10.1 antibody, si:ch211-254j6.1 antibody, si:ch211-278f21.4 antibody, wu:fc31a09 antibody, fk52c05 antibody, flk antibody, kdr antibody, kdra antibody, vegfr2 antibody, vegfr4 antibody, vegr2 antibody, wu:fk52c05 antibody, kinase insert domain receptor antibody, kinase insert domain protein receptor antibody, vascular endothelial growth factor receptor kdr-like antibody, kinase insert domain receptor (a type III receptor tyrosine kinase) antibody, kinase insert domain receptor like antibody, KDR antibody, Kdr antibody, LOC100136647 antibody, kdr antibody, kdrl antibody

Background: Synonyms: Fetal liver kinase 1, CD309, Kdr, VEGF receptor-2, VEGFR-2

Pathways: RTK Signaling, Glycosaminoglycan Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling