H2-D1 antibody (Biotin)

Catalog No. ABIN2689536

Product Details anti-H2-D1 Antibody (Biotin)

Target: H2-D1 (Histocompatibility 2, D Region Locus 1 (H2-D1))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This H2-D1 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Characteristics: The 28-14-8 antibody reacts with the α3 domain of the H-2D[b] MHC class I alloantigen. The antibody binds to H-2D[b] in the presence or absence of the β2 microglobulin chain. It cross-reacts with the α3 domain of H-2L[d], but not with K[d] or D[d], and with H-2D[q] and/or L[q]. Reactivity with haplotypes k, f, p, r, and s has not been observed. mAb 28-14-8 has been shown to block H-2L[d]- specific and H- 2L[d]-restricted antigen-specific lysis of target cells by cytotoxic T lymphocytes (CTL), but it does not block recognition of H-2L[d] positive target cells by Ly-6G2-positive NK cells.

BD Pharmingen™ Biotin Mouse Anti-Mouse H-2D[b] - Biotin - Clone 28-14-8 - Isotype Mouse IgG2a, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: C3H.SW mouse splenocytes

Clone: 28-14

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-H2-D1 antibody (Biotin) (ABIN2689536)

Kündig, Bachmann, DiPaolo, Simard, Battegay, Lother, Gessner, Kühlcke, Ohashi, Hengartner: "Fibroblasts as efficient antigen-presenting cells in lymphoid organs." in: Science (New York, N.Y.), Vol. 268, Issue 5215, pp. 1343-7, (1995) (PubMed).

Mason, Ortaldo, Young, Kumar, Bennett, Anderson: "Cloning and functional characteristics of murine large granular lymphocyte-1: a member of the Ly-49 gene family (Ly-49G2)" in: The Journal of experimental medicine, Vol. 182, Issue 2, pp. 293-303, (1995) (PubMed).

Allen, Fraser, Flyer, Calvin, Flavell: "Beta 2-microglobulin is not required for cell surface expression of the murine class I histocompatibility antigen H-2Db or of a truncated H-2Db." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 19, pp. 7447-51, (1986) (PubMed).

Allen, Wraith, Pala, Askonas, Flavell: "Domain interactions of H-2 class I antigens alter cytotoxic T-cell recognition sites." in: Nature, Vol. 309, Issue 5965, pp. 279-81, (1984) (PubMed).

Evans, Margulies, Shykind, Seidman, Ozato: "Exon shuffling: mapping polymorphic determinants on hybrid mouse transplantation antigens." in: Nature, Vol. 300, Issue 5894, pp. 755-7, (1983) (PubMed).

Orn, Goodenow, Hood, Brayton, Woodward, Harmon, Frelinger: "Product of a transferred H-2Ld gene acts as restriction element for LCMV-specific killer T cells." in: Nature, Vol. 297, Issue 5865, pp. 415-7, (1982) (PubMed).

Woodward, Orn, Harmon, Goodenow, Hood, Frelinger: "Specific recognition of the product of a transferred major histocompatibility complex gene by cytotoxic T lymphocytes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 79, Issue 11, pp. 3613-7, (1982) (PubMed).

Ozato, Hansen, Sachs: "Monoclonal antibodies to mouse MHC antigens. II. Antibodies to the H-2Ld antigen, the products of a third polymorphic locus of the mouse major histocompatibility complex." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 125, Issue 6, pp. 2473-7, (1981) (PubMed).

Ozato, Sachs: "Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 126, Issue 1, pp. 317-21, (1981) (PubMed).

Target Details for H2-D1

Target: H2-D1 (Histocompatibility 2, D Region Locus 1 (H2-D1))

Alternative Name: H-2D[b] (H2-D1 Products)

Synonyms: H-2D antibody, H2-D antibody, histocompatibility 2, D region locus 1 antibody, H2-D1 antibody

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process