MHC Class II I-Ek antibody

Catalog No. ABIN2689579

Product Details anti-MHC Class II I-Ek Antibody

Target: MHC Class II I-Ek

Reactivity: Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This MHC Class II I-Ek antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Cytotoxicity Test (CyTox), Blocking Reagent (BR)

Brand: BD Pharmingen™

Characteristics: The 14-4-4S antibody reacts with the I-E[k] MHC class II alloantigen. It cross-reacts with cells from mice of the H-2[d], H-2[p], and H-2[r] haplotypes. Cells from mice of the H-2[b], H-2[f], H-2[g7], H-2[q], and H-2[s] haplotypes do not express I-E antigen. It has been reported that mAb 14-4-4S cross-reacts with the rat MHC class II antigen RT1D. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Purified Mouse Anti-Mouse I-E[k] - Purified - Clone 14-4-4S - Isotype Mouse IgG2a, κ - Reactivity Ms, Rat - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: C3H mouse skin graft and splenocytes

Clone: 14-4

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-MHC Class II I-Ek antibody (ABIN2689579)

Grakoui, Bromley, Sumen, Davis, Shaw, Allen, Dustin: "The immunological synapse: a molecular machine controlling T cell activation." in: Science (New York, N.Y.), Vol. 285, Issue 5425, pp. 221-7, (1999) (PubMed).

Roy, Aruffo, Ledbetter, Linsley, Kehry, Noelle: "Studies on the interdependence of gp39 and B7 expression and function during antigen-specific immune responses." in: European journal of immunology, Vol. 25, Issue 2, pp. 596-603, (1995) (PubMed).

Neiss, Reske: "Non-coordinate synthesis of MHC class II proteins and invariant chains by epidermal Langerhans cells derived from short-term in vitro culture." in: International immunology, Vol. 6, Issue 1, pp. 61-71, (1994) (PubMed).

Harton, Litaker, Frelinger, Bishop: "Structure function analysis of the H-2 Abp gene." in: Immunogenetics, Vol. 34, Issue 6, pp. 358-65, (1992) (PubMed).

Hattori, Buse, Jackson, Glimcher, Dorf, Minami, Makino, Moriwaki, Kuzuya, Imura: "The NOD mouse: recessive diabetogenic gene in the major histocompatibility complex." in: Science (New York, N.Y.), Vol. 231, Issue 4739, pp. 733-5, (1986) (PubMed).

Blankenhorn, Symington, Cramer: "Biochemical characterization of Ia antigens encoded by the RT1.B and RT1.D loci in the rat MHC." in: Immunogenetics, Vol. 17, Issue 5, pp. 475-84, (1983) (PubMed).

Bhattacharya, Dorf, Springer: "A shared alloantigenic determinant on Ia antigens encoded by the I-A and I-E subregions: evidence for I region gene duplication." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 127, Issue 6, pp. 2488-95, (1982) (PubMed).

Ozato, Mayer, Sachs: "Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 124, Issue 2, pp. 533-40, (1980) (PubMed).

Target Details for MHC Class II I-Ek

Target: MHC Class II I-Ek

Alternative Name: I-E[k] (MHC Class II I-Ek Products)