IL-5 antibody (Biotin)

Catalog No. ABIN2689774

Product Details anti-IL-5 Antibody (Biotin)

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-5 antibody is conjugated to Biotin

Application: ELISA

Brand: BD Pharmingen™

Characteristics: The TRFK4 antibody reaacts with mouse interleukin-5 (IL-5). The immunogen used to generate the TRFK4 hybridoma was a semi-purified T cell clone supernatant. This is a neutralizing antibody.

BD Pharmingen™ Biotin Rat Anti-Mouse IL-5 - Biotin - Clone TRFK4 - Isotype Rat IgG2a - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Semi-purified T Cell

Clone: TRFK4

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-IL-5 antibody (Biotin) (ABIN2689774)

Abrams: "Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies." in: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 6, pp. Unit 6.20, (2008) (PubMed).

Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, (1992) (PubMed).

Sander, Andersson, Andersson: "Assessment of cytokines by immunofluorescence and the paraformaldehyde-saponin procedure." in: Immunological reviews, Vol. 119, pp. 65-93, (1991) (PubMed).

Schumacher, OGarra, Shrader, van Kimmenade, Bond, Mosmann, Coffman: "The characterization of four monoclonal antibodies specific for mouse IL-5 and development of mouse and human IL-5 enzyme-linked immunosorbent." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 141, Issue 5, pp. 1576-81, (1988) (PubMed).

Target Details for IL-5

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Alternative Name: IL-5 (IL5 Products)

Synonyms: EDF antibody, IL-5 antibody, TRF antibody, Il-5 antibody, IL5 antibody, interleukin 5 antibody, IL5 antibody, Il5 antibody

Pathways: JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour