Klra4 antibody
Klra4
Reactivity: Mouse
FACS, BR, IP
Host: Rat
Monoclonal
4E5
unconjugated
(1 reference)-
- Target See all Klra4 products
- Klra4 (Killer Cell Lectin-Like Receptor, Subfamily A, Member 4 (Klra4))
- Reactivity
- Mouse
- Host
- Rat
- Clonality
- Monoclonal
- Application
- Flow Cytometry (FACS), Blocking Reagent (BR), Immunoprecipitation (IP)
- Brand
- BD Pharmingen™
- Characteristics
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The 4E5 antibody reacts with Ly-49D, which is expressed on subsets of natural killer (NK) cells in C57BL/6, C3H/He (at a very low frequency), and SJL, but not DBA/2, AKR, CBA/J, or BALB/c mice. Unlike Ly-49D antigen has not been betected on NK-1.1+ (or DX5+) T cells. In 129/J mice, the 4E5 antibody cross-reacts with Ly-49O, Ly-49R, and Ly-49V. The Ly-49 family of NK-cell receptors, members of the C-type lectin superfamily, are disulfide-linked type-II transmembrane protein homodimers with extracellular carbohydrate-recognition domains, which bind to MHC class I alloantigens. The Ly-49 family members are expressed independently, such that an individual NK or T cell may display more than one class of Ly-49 receptor homodimers. Ly-49D weakly binds to MHC class I antigens of the k halpotype, and Ly-49D+ IL-2-activated NK cells lyse target cells expressing H-2[a], H-2[b], H-2[d], H-2[k], H-2[p], H-2[q], and H-2[s] and the CHO (Chinese hamster ovary) cell line. Ly-49D+ cells mediate allogenic resistance to H-2d bone marrow transplantation. In vitro studies suggest that the Ly-49D receptor mediates activation of NK-cell cytolytic activity via tyrosine phosphorylation of their ITIMs (Immunoreceptor Tyrosine-based Inhibitory Motifs). Molecular differences between the Ly-49D stimulatory receptor and the inhibitory members of the Ly-49 family include the absence of an ITIM in Ly-49D, the lack of phosphorylation of Ly-49D in activated NK cells, and the association of a novel tyrosine-phosphorylated protein (pp16) with Ly-49D in activated NK cells. Ly-49O and Ly-49V are closely related to Ly-49A[B6] and, like Ly-49A, have ITIM domains. Ly-49O- and Ly-49V-transfected 293T (human kidney epithelial) cells bind tetramers of H-2D[b], D[b], D[k], and L[d]. In addition, the Ly-49V-transfected cells also bind K[b], K[d], and K[k]. Ly-49R is closely related to Ly-49D[B6] and is putative activating receptor due to its lack of an ITIM domain. Ly-49R-transfected 293T cells bind soluble tetramers of H-2D[b], D[d], D[k], and L[d]. Two-color analysis of Ly-49D expression on splenic NK cells. C57Bl/6 splenocytes were simultaneously stained with PE-conjugated mAb PK136 (anti-mouse NK-1.1, Cat. No. 557391/553165, both panels) and purified 4E5 mAb (right panel), followed by FITC-conjugated goat anti-rat Ig (Cat. No. 554016, both panels). Flow cytometry was performed on a FACScan™ .
BD Pharmingen™ Purified Rat Anti-Mouse Ly-49D - Purified - Clone 4E5 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg - Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogen
- Not Reported
- Clone
- 4E5
- Isotype
- IgG2a kappa
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Store undiluted at 4°C.
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: "Direct assessment of MHC class I binding by seven Ly49 inhibitory NK cell receptors." in: Immunity, Vol. 11, Issue 1, pp. 67-77, (1999) (PubMed).
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: "Direct assessment of MHC class I binding by seven Ly49 inhibitory NK cell receptors." in: Immunity, Vol. 11, Issue 1, pp. 67-77, (1999) (PubMed).
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- Target
- Klra4 (Killer Cell Lectin-Like Receptor, Subfamily A, Member 4 (Klra4))
- Alternative Name
- Ly-49D (Klra4 Products)
- Synonyms
- Chok antibody, Klra32 antibody, Ly-49d antibody, Ly49d antibody, ly49r<129> antibody, killer cell lectin-like receptor, subfamily A, member 4 antibody, Klra4 antibody
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