MICA/B antibody

Catalog No. ABIN2689844

Product Details anti-MICA/B Antibody

Target: MICA/B (MICA/MICB) (MHC Class I Polypeptide-Related Sequence A/B (MICA/MICB))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MICA/B antibody is un-conjugated

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Characteristics: Monoclonal antibody 6D4 reacts with MICA and MICB, distant homologs of major histocompatibility complex class I proteins. They are highly glycosylated molecules of approximately 70 kDa that are normally expressed in gut epithelium, are frequently associated with epithelial tumors and can be induced by some viral and bacterial infections. MIC are highly expressed on proliferating but not on quiescent epithelial cell lines, on which they can be heat-shock induced. MIC have similar functional and structural properties and are ligands for NKG2D, an activating receptor on most NK cells, CD8 T cells and ?d T cells. Expression of MIC has been observed on synoviocytes in rheumatoid arthritis and on epithelial cells in transplanted kidney and pancreas showing histological signs of rejection and or cellular injury. Profile of anti MIC A/B (clone 6D4) reactivity on HeLa cells analyzed by flow cytometry. Second step staining with Cat. No. 550589.

BD Pharmingen™ Purified Anti-Human MIC A/B - Purified - Clone 6D4 - Isotype Mouse IgG2a, κ - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Transfected cells

Clone: 6D4

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-MICA/B antibody (ABIN2689844)

Groh, Bruhl, El-Gabalawy, Nelson, Spies: "Stimulation of T cell autoreactivity by anomalous expression of NKG2D and its MIC ligands in rheumatoid arthritis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 16, pp. 9452-7, (2003) (PubMed).

Hankey, Drachenberg, Papadimitriou, Klassen, Philosophe, Bartlett, Groh, Spies, Mann: "MIC expression in renal and pancreatic allografts." in: Transplantation, Vol. 73, Issue 2, pp. 304-6, (2002) (PubMed).

Das, Groh, Kuijl, Sugita, Morita, Spies, Bukowski: "MICA engagement by human Vgamma2Vdelta2 T cells enhances their antigen-dependent effector function." in: Immunity, Vol. 15, Issue 1, pp. 83-93, (2001) (PubMed).

Groh, Rhinehart, Randolph-Habecker, Topp, Riddell, Spies: "Costimulation of CD8alphabeta T cells by NKG2D via engagement by MIC induced on virus-infected cells." in: Nature immunology, Vol. 2, Issue 3, pp. 255-60, (2001) (PubMed).

Bauer, Groh, Wu, Steinle, Phillips, Lanier, Spies: "Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA." in: Science (New York, N.Y.), Vol. 285, Issue 5428, pp. 727-9, (1999) (PubMed).

Groh, Rhinehart, Secrist, Bauer, Grabstein, Spies: "Broad tumor-associated expression and recognition by tumor-derived gamma delta T cells of MICA and MICB." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 12, pp. 6879-84, (1999) (PubMed).

Target Details for MICA/B

Target: MICA/B (MICA/MICB) (MHC Class I Polypeptide-Related Sequence A/B (MICA/MICB))

Alternative Name: MIC A/B (MICA/MICB Products)