MLH1 antibody

Catalog No. ABIN2689847

Product Details anti-MLH1 Antibody

Target: MLH1 (MutL Homolog 1 (MLH1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MLH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Brand: BD Pharmingen™

Characteristics: The repair of mismatched DNA is essential to maintaining the integrity of genetic information over time. In bacteria the DNA repair process is accomplished by the MutL, MutH, and MutS proteins. The MutS protein initially recognizes and binds to mismatched DNA. Following this, MutH, an endonuclease, and MutL form a complex with MutS and carry out an excision repair mechanism. When bacteria are deficient in one of these enzymes a mutator phenotype arises characterized by genetic instability. The important role played by DNA repair enzymes is emphasized by the fact that they are highly conserved from bacteria to yeast to mammals. In yeast the proteins are called MutS homolog 2 (MSH2), MutL homolog (MLH1), and PMS1 which is also a homolog of MutL. MSH2 is involved in the initial recognition of mismatched nucleotides during the replication mismatch repair process. It is thought that after MSH2 binds to a mismatched DNA duplex it is joined by a heterodimer of MLH1 and PMS1 which together help facilitate the later steps in mismatch repair. Biochemical studies of the human homologs of DNA mismatch repair enzymes MLH1, PMS2, and MSH2 indicate that human MSH2 protein can bind mispaired DNA, and that human MLH1 and PMS2 can exist as a heterodimer. These and other studies support the conservation of eukaryotic DNA mismatch repair mechanisms. The G168-15 antibody recognizes human MLH1 (80-85 kDa). Full-length human recombinant MLH was expressed as a fusion protein, affinity purified, and used as immunogen. Immunohistochemistry of MLH-1 in human colon carcinoma. Frozen sections of human colon carcinoma were reacted with the MLH-1 antibody. Cells expressing MLH-1 can be identifed by the intense brown labeling of their cell nuclei. Magnification 20X.

BD Pharmingen™ Purified Mouse Anti-Human MLH-1 - Purified - Clone G168-15 - Isotype Mouse IgG1 - Reactivity Hu - 1 mL

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human recombinant MLH

Clone: G168

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-MLH1 antibody (ABIN2689847)

Baker, Plug, Prolla, Bronner, Harris, Yao, Christie, Monell, Arnheim, Bradley, Ashley, Liskay: "Involvement of mouse Mlh1 in DNA mismatch repair and meiotic crossing over." in: Nature genetics, Vol. 13, Issue 3, pp. 336-42, (1996) (PubMed).

Cleaver: "It was a very good year for DNA repair." in: Cell, Vol. 76, Issue 1, pp. 1-4, (1994) (PubMed).

Prolla, Christie, Liskay: "Dual requirement in yeast DNA mismatch repair for MLH1 and PMS1, two homologs of the bacterial mutL gene." in: Molecular and cellular biology, Vol. 14, Issue 1, pp. 407-15, (1994) (PubMed).

Prolla, Pang, Alani, Kolodner, Liskay: "MLH1, PMS1, and MSH2 interactions during the initiation of DNA mismatch repair in yeast." in: Science (New York, N.Y.), Vol. 265, Issue 5175, pp. 1091-3, (1994) (PubMed).

Su, Modrich: "Escherichia coli mutS-encoded protein binds to mismatched DNA base pairs." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 14, pp. 5057-61, (1986) (PubMed).

Target Details for MLH1

Target: MLH1 (MutL Homolog 1 (MLH1))

Alternative Name: MLH-1 (MLH1 Products)

Synonyms: CG11482 antibody, Dmel\\CG11482 antibody, dmlh-1 antibody, dmlh1 antibody, zgc:66301 antibody, MLH1 antibody, LOC100232198 antibody, 1110035C23Rik antibody, AI317206 antibody, AI325952 antibody, AI561766 antibody, COCA2 antibody, FCC2 antibody, HNPCC antibody, HNPCC2 antibody, hMLH1 antibody, mutL homolog 1 antibody, CG11482 gene product from transcript CG11482-RB antibody, mutL homolog 1 S homeolog antibody, mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) antibody, DNA mismatch repair protein Mlh1 antibody, MLH1 antibody, Mlh1 antibody, mlh1.S antibody, mlh1 antibody, LOC588545 antibody

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response