T Cell Receptor (TCR) V beta 8 antibody (Biotin)
Reactivity: Mouse
IHC (fro), FACS
Host: Mouse
Monoclonal
F23-1
Biotin
(15 references)-
- Target
- T Cell Receptor (TCR) V beta 8
- Reactivity
- Mouse
- Host
- Mouse
- Clonality
- Monoclonal
- Conjugate
- Biotin
- Application
- Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)
- Brand
- BD Pharmingen™
- Characteristics
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The F23.1 antibody reacts with the Vβ 8.1, Vβ 8.2, and Vβ 8.3 T-cell receptors (TCR) of mice having the b haplotype (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C58, DBA/1, DBA/2) of the Tcrb gene complex. The Tcrb-V8 subfamily gene loci are deleted in mice having the a (e.g., C57BR, C57L, SJL, SWR) or c (e.g., RIII) haplotype. Vβ 8.1 TCR-bearing T lymphocytes are clonally eliminated in mice expressing superantigen coded by Mtv-7 (Mls-1a, Mlsa) provirus (e.g., AKR, CBA/J, C58, DBA/2), and activation or elimination of Vβ 8.1 TCR-expressing T cells by this determinant is partially dependent upon presentation by I-E. Mtv-43 and/or exogenous MMTV-SW superantigens also cause incomplete elimination of Vβ 8.1 TCR-bearing T cells. In addition to expression on conventional T lymphocytes, Vβ 8.2 is the predominant β chain of the TCR on NK-T cells. Staphylococcal enterotoxin B, in association with antigen-presenting cells expressing I-A and/or I-E, stimulates lymphocytes bearing Vβ 8 TCR and selectively eliminates those T cells in vivo. Soluble and plate-bound F23.1 antibody activates Vβ 8 TCR-bearing T cells, soluble antibody blocks cytolysis mediated by Vβ 8 TCR-bearing cytotoxic T lymphocytes, and in vivo treatment of neonatal mice can arrest intrathymic maturation of Vβ 8 TCR-bearing T cells. Two-color analysis of the expression of Vβ 8 TCR on peripheral lymphocytes. C57BL/6 lymph node cells were incubated simultaneously with biotin-conjugated F23.1, PE-conjugated RM4-5 (anti-CD4, Cat. No. 553048/553049), and PE-conjugated 53-6.7 (anti-CD8a, Cat. No. 553032/553033) monoclonal antibodies, followed by Avidin-FITC (Cat. No. 554057). Flow cytometry was performed on a FACScan™ 9BDIS, San Jose, CA).
BD Pharmingen™ Biotin Mouse Anti-Mouse Vβ 8 T-Cell Receptor - Biotin - Clone F23.1 - Isotype Mouse IgG2a, κ - Reactivity Ms - 0.25 mg - Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogen
- BALB.B Mouse Lymph-Node and Spleen T Cells
- Clone
- F23-1
- Isotype
- IgG2a kappa
-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
- Storage
- 4 °C
- Storage Comment
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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: "Fas (CD95) participates in peripheral T cell deletion and associated apoptosis in vivo." in: International immunology, Vol. 7, Issue 9, pp. 1451-8, (1996) (PubMed).
: "Peripheral T cells undergoing superantigen-induced apoptosis in vivo express B220 and upregulate Fas and Fas ligand." in: The Journal of experimental medicine, Vol. 183, Issue 2, pp. 431-7, (1996) (PubMed).
: "Mouse NK1+ T cells." in: Current opinion in immunology, Vol. 7, Issue 3, pp. 367-74, (1995) (PubMed).
: "Thymic epithelial cell lines that mediate positive selection can also induce thymocyte clonal deletion." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 3, pp. 1022-31, (1994) (PubMed).
: "Superantigen-like properties of an antibody bispecific for MHC class II molecules and the V beta domain of the T cell antigen receptor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 8, pp. 3833-41, (1994) (PubMed).
: "TCR-CD4 and TCR-TCR interactions as distinctive mechanisms for the induction of increased intracellular calcium in T-cell signalling." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 3, pp. 1337-45, (1993) (PubMed).
: "Clonal expansion precedes anergy and death of V beta 8+ peripheral T cells responding to staphylococcal enterotoxin B in vivo." in: European journal of immunology, Vol. 21, Issue 8, pp. 1963-6, (1991) (PubMed).
: "RIII S/J (H-2r). An inbred mouse strain with a massive deletion of T cell receptor V beta genes." in: The Journal of experimental medicine, Vol. 169, Issue 6, pp. 1903-9, (1989) (PubMed).
: "Antibodies against the T cell receptor/CD3 complex interfere with distinct intra-thymic cell-cell interactions in vivo: correlation with arrest of T cell differentiation." in: European journal of immunology, Vol. 19, Issue 5, pp. 857-63, (1989) (PubMed).
: "The V beta-specific superantigen staphylococcal enterotoxin B: stimulation of mature T cells and clonal deletion in neonatal mice." in: Cell, Vol. 56, Issue 1, pp. 27-35, (1989) (PubMed).
: "In situ localization of T cell receptor beta chain in the murine thymus: changes in the intrathymic distribution of thymocytes expressing beta chain during fetal development." in: Cellular immunology, Vol. 116, Issue 1, pp. 135-48, (1988) (PubMed).
: "Self-tolerance eliminates T cells specific for Mls-modified products of the major histocompatibility complex." in: Nature, Vol. 332, Issue 6159, pp. 35-40, (1988) (PubMed).
: "Expression of a murine polyclonal T cell receptor marker correlates with the use of specific members of the V beta 8 gene segment subfamily." in: The Journal of experimental medicine, Vol. 165, Issue 1, pp. 257-62, (1987) (PubMed).
: "Murine T-cell receptor mutants with deletions of beta-chain variable region genes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 3, pp. 767-71, (1986) (PubMed).
: "Characterization of a murine monoclonal antibody specific for an allotypic determinant on T cell antigen receptor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 134, Issue 6, pp. 3994-4000, (1985) (PubMed).
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: "Fas (CD95) participates in peripheral T cell deletion and associated apoptosis in vivo." in: International immunology, Vol. 7, Issue 9, pp. 1451-8, (1996) (PubMed).
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- Target
- T Cell Receptor (TCR) V beta 8
- Alternative Name
- Vbeta 8 T-Cell Receptor
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