MAP1B antibody (Microtubule-Associated Protein 1B)

Details for Product anti-MAP1B Antibody No. ABIN269612, Supplier: Log in to see
Antigen
  • FUTSCH
  • MAP5
  • Mtap1b
  • A230055D22
  • AI843217
  • LC1
  • Mtap-5
  • Mtap5
  • microtubule associated protein 1B
  • microtubule-associated protein 1B
  • MAP1B
  • Map1b
Alternatives
anti-Cow (Bovine) MAP1B antibody for Immunocytochemistry
Reactivity
Cow (Bovine), Chicken, Cat (Feline), Human, Mouse (Murine), Rat (Rattus)
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60
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2
2
1
Host
Mouse
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13
Clonality (Clone)
Monoclonal ()
Conjugate
This MAP1B antibody is un-conjugated
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3
3
3
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2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
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1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (IHC), Microarray (MA), Western Blotting (WB)
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37
26
14
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Options
Supplier
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Supplier Product No.
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Immunogen Rat brain microtubule associated proteins.
Clone AA6
Isotype IgG1
Specificity The antibody does not cross-react with other MAPs or tubulin.
Purification Unpurified
Plasmids, Primers & others Plasmids, Primers & others MAP1B products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name MAP1B (MAP1B Antibody Abstract)
Background Gene Symbol: MAP1B
Molecular Weight Theoretical MW: 271 kDa
Gene ID 4131
UniProt P15205
Pathways Microtubule Dynamics, Regulation of Cell Size
Application Notes Western Blot 1:500, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500, MicroarrayThe observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for MAP1B Antibody IHC protocol for cryostat sections (Protocol generously provided by Dr. Yimin Wang, Tissue Regeneration Therapeutics Inc )
1. Take out the cryostat section slides from -80C. Dry for few minutes at RT.
. Rinse slides with PBS to get rid of OCT. 3 min x 2, shake
. Fix slides with 1:1 methanol:acetone for 3 min
. Wash slides with PBS, 3 min x
. Circle tissue sections with PAP-Pen,
. Incubation with Normal Horse Serum: 5 % in 2 %BSA (in PBS-Tween20) for 20 minutes.
. MAP1B anti human antibody (1:200 in 2 % BSA 0.1 % Tween20). Incubate for 1.5 hours at 37C in a humid chamber.
. Wash slides with PBS-Tween (0.1 %), 5min x2The following steps are for IHC only
. Blocking of endogenous peroxidase: Incubation with 0.3 % H2O2 for 10 minutes.
. Wash PBS-Tween (0.1 %), 3 min x
. Incubation with secondary antibody (1:200 with PBS, anti mouse-bio, in AK-5002 kit) for 45 minutes at 37C in a humid chamber.
. Wash slides with PBS-Tween (0.1 %) 5min x
. Incubate with VECTAINABC reagent solution (bottle C, Cat No: PK6100, contains Avidin-HRP) for 30 minutes at RT in the dark.(For solution C: 10ml PBS + 2 drops of A and 2 drops of B, kept in 4C with dark)Prewarm at RT for 10min before use and drop 2 drops to the sections.
. Wash slides with PBS-Tween (0.1 %) 5min x
. Incubation with peroxidase substrate (Vector Nova Red, SK-4800) until desired stain intensity develops. Usually 1 to 2 minutes.Prepare the substrate immediately before use: can be used for > 1week.- Add 3 drops of Reagent 1 to 5 mL of DDW, mix well- Add 2 drops of Reagent 2 and mix well- Add 2 drops of Reagent 3 and mix well- Add 2 drops of Hydrogen Peroxide Solution and mix well
. Wash slides with tap water for 5 minutes.
. Hematoxylin nuclear staining: (Filter solution when first use, HE staining Jar,2nd one). Stain slides for 5 seconds at room temperature, then carefully rinse for 3 minutes under running cold tap water,
. Mount the slide with cc mount (Sigma, C9368, 30ml)
Restrictions For Research Use only
Format Liquid
Buffer Ascites
Buffer contains: 15 mM Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.
Supplier Images
Immunohistochemistry (IHC) image for anti-Microtubule-Associated Protein 1B (MAP1B) antibody (ABIN269612) Immunohistochemistry: MAP1B Antibody (AA6) [ABIN269612] - MAP1B expression on human u...
Immunofluorescence (IF) image for anti-Microtubule-Associated Protein 1B (MAP1B) antibody (ABIN269612) Immunocytochemistry/Immunofluorescence: MAP1B Antibody (AA6) [ABIN269612] - Staining ...
Product cited in: Kaneko, Arayatrakoollikit, Yamanaka, Ito, Okiji: "Immunohistochemical and gene expression analysis of stem-cell-associated markers in rat dental pulp." in: Cell and tissue research, Vol. 351, Issue 3, pp. 425-32, 2013 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunohistochemistry

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