TUBB antibody (Tubulin, beta)

Details for Product anti-TUBB Antibody No. ABIN269677
Antigen
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Primate, Rabbit, Rat (Rattus)
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Host
Mouse
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Clonality (Clone)
Monoclonal ()
Conjugate
This TUBB antibody is un-conjugated
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Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
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Options
Immunogen Sea urchin (Lytechinus pictus) sperm axonemal proteins.
Clone D66
Isotype IgG1
Specificity This antibody recognizes all mouse brain beta tubulin isoforms (beta and beta subpopulations), and sea urchin beta Tubulin.
Purification Protein A or G purified
Antigen
Alternative Name beta Tubulin (TUBB Antibody Abstract)
Background Gene Symbol: TUBB
Gene ID 203068
Pathways Microtubule Dynamics, M Phase
Application Notes Western Blot 0.5-1 μg/mL, ELISA 1:100-1:2000, Immunocytochemistry/Immunofluorescence 1:10-1:2000, Immunoprecipitation 1:10-1:500ICC:(3 % paraformaldehyde-0.5 % Triton X-100 and methanol-acetone. Predicted molecular weight: 50 kDa.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer 10 mM PBS ( pH 7.4) and 1.0 % BSA
Buffer contains: 15 mM Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Image no. 1 for anti-Tubulin, beta (TUBB) antibody (ABIN269677) Western Blot: beta Tubulin Antibody (D66) [ABIN269677] - beta Tubulin antibody Total ...
Product cited in: Jansen, Mirzaa, Ishak, ORoak, Hiatt, Roden, Gunter, Christian, Collins, Adams, Rivière, St-Onge, Ojemann, Shendure, Hevner, Dobyns: "PI3K/AKT pathway mutations cause a spectrum of brain malformations from megalencephaly to focal cortical dysplasia." in: Brain : a journal of neurology, Vol. 138, Issue Pt 6, pp. 1613-28, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Jansen, Hevner, Roden, Hahn, Jung, Gospe: "Glial localization of antiquitin: implications for pyridoxine-dependent epilepsy." in: Annals of neurology, Vol. 75, Issue 1, pp. 22-32, 2014 (PubMed). (Sample species: Human). Further details: Western Blotting