TGFBI antibody (cleaved, N-Term)

Catalog No. ABIN2712086

Product Details anti-TGFBI Antibody

Target: TGFBI (Transforming Growth Factor, beta-Induced, 68kDa (TGFBI))

Binding Specificity: cleaved, N-Term

Reactivity: Arabidopsis thaliana, Oryza sativa

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TGFBI antibody is un-conjugated

Application: Western Blotting (WB)

Characteristics: Expected / apparent Molecular Weight of the Antigene: 22 / 19 kDa (Arabidopsis thaliana)

Purification: serum

Immunogen: overexpressed Arabidopsis thaliana Cu/ZnSOD O78310, At2g28190 with an N-terminal His-tag. Purified via Ni-column. The His-tag was cleaved and the protein was recovered via ion exchange on a 10 ml resource-Q (Pharmacia) column using a NaCl gradient. Pure fractions after dialysis against PBS has been used for immunization.

Application Details

Application Notes: Recommended Dilution: 1 : 1000 alkaline phosphatase (WB).

Comment:

Note: Antibody is recognizing 19 kDa protein Csd2 (chloroplastic enzyme) and gives also low but noticeable reactivity to Csd1 (15 kDa) a cytosolic form

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 µL of sterile water.

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

References for anti-TGFBI antibody (ABIN2712086)

Speth, Willing, Rausch, Schneeberger, Laubinger: "RACK1 scaffold proteins influence miRNA abundance in Arabidopsis." in: The Plant journal : for cell and molecular biology, Vol. 76, Issue 3, pp. 433-45, (2013) (PubMed).

Yu, Xu, Zheng, Wang: "Comparative proteomic study reveals the involvement of diurnal cycle in cell division, enlargement, and starch accumulation in developing endosperm of Oryza sativa." in: Journal of proteome research, Vol. 11, Issue 1, pp. 359-71, (2012) (PubMed).

Target Details for TGFBI

Target: TGFBI (Transforming Growth Factor, beta-Induced, 68kDa (TGFBI))

Alternative Name: CSD2 (TGFBI Products)

Synonyms: TGFBI antibody, BIGH3 antibody, CDB1 antibody, CDG2 antibody, CDGG1 antibody, CSD antibody, CSD1 antibody, CSD2 antibody, CSD3 antibody, EBMD antibody, LCD1 antibody, 68kDa antibody, AI181842 antibody, AI747162 antibody, Beta-ig antibody, big-h3 antibody, Big-h3 antibody, MP70 antibody, MP78 antibody, hm:zeh0933 antibody, wu:fa13f05 antibody, zgc:110495 antibody, transforming growth factor beta induced antibody, transforming growth factor, beta induced antibody, transforming growth factor, beta-induced antibody, TGFBI antibody, Tgfbi antibody, tgfbi antibody

Background: AGI Code: At2g28190
Antioxidant system works as a defense against oxidative stress. SOD (superoxide dismutase) catalyzes the dismutation of superoxide into oxygen and H202,. SODs are classified, according to their metal cofactor, as FeSOD, MnSOD, or Cu / ZnSOD. Chloroplasts generally contain Cu/ZnSOD and, in a number of plant species, FeSOD

Molecular Weight: expected: 22 kDa, apparent: 19 kDa (Arabidopsis thaliana)

UniProt: O78310