USF2 antibody

Catalog No. ABIN271975

Product Details anti-USF2 Antibody

Target: USF2 (Upstream Transcription Factor 2, C-Fos Interacting (USF2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This USF2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of USF2 protein. (region surrounding Asp226/159)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for USF2

Target: USF2 (Upstream Transcription Factor 2, C-Fos Interacting (USF2))

Alternative Name: USF2 (USF2 Products)

Synonyms: fip antibody, MGC79773 antibody, bhlhb12 antibody, USF2 antibody, Usf-2 antibody, bHLHb12 antibody, FIP antibody, fb12g11 antibody, si:dkey-211g8.3 antibody, wu:fb12g11 antibody, usf2 antibody, upstream transcription factor 2, c-fos interacting antibody, upstream transcription factor 2, c-fos interacting S homeolog antibody, upstream stimulatory factor 2 antibody, upstream transcription factor 2 antibody, upstream transcription factor 2, c-fos interacting L homeolog antibody, usf2 antibody, usf2.S antibody, USF2 antibody, Tsp_04848 antibody, Usf2 antibody, usf2.L antibody

Background: The ubiquitously expressed cellular upstream stimulatory factor (USF) consists of 43 kDa (USF-1) and 44 kDa (USF-2) polypeptides which independently exhibit site-specific DNA binding and are members of the c-Myc-related family of regulatory factors containing helix-loop-helix domains. USF also contains a leucine repeat that is required for efficient DNA binding. USF was originally identified as an upstream stimulatory factor that binds the core sequence CACGTG in the adenovirus late promoter. These findings, together with the demonstration of cooperative interaction between USF and the initiator-binding protein TFII-I, raise the possibility of a more general involvement of USF in transcriptional regulation. While expression of both USF-1 and USF-2 species is ubiquitous, different ratios of USF homo- and hetero-dimers are found in different cell types.Synonyms: BHLHB12, Class B basic helix-loop-helix protein 12, FIP, FOS-interacting protein, Major late transcription factor 2, Upstream stimulatory factor 2, Upstream transcription factor 2

Molecular Weight: approx. 36 kDa

Gene ID: 7392

NCBI Accession: NP_003358

UniProt: Q15853