COL3A1 antibody (Chain alpha 1)

Catalog No. ABIN272016

Product Details anti-COL3A1 Antibody

Target: COL3A1 (Collagen, Type III, alpha 1 (COL3A1))

Binding Specificity: Chain alpha 1

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COL3A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody detects endogenous levels of Collagen III protein (COL3A1).

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen

Application Details

Application Notes: ELISA: 1/10000approx. 1/20000. Immunoflourescence: 1/50approx. 1/200. Immunohistochemistry: 1/50approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-COL3A1 antibody (ABIN272016)

Xie, Dolder, Siegrist, Wetterwald, Hofstetter: "Glutamate Receptor Agonists and Glutamate Transporter Antagonists Regulate Differentiation of Osteoblast Lineage Cells." in: Calcified tissue international, Vol. 99, Issue 2, pp. 142-54, (2018) (PubMed).

Schminke, Frese, Bode, Goldring, Miosge: "Laminins and Nidogens in the Pericellular Matrix of Chondrocytes: Their Role in Osteoarthritis and Chondrogenic Differentiation." in: The American journal of pathology, Vol. 186, Issue 2, pp. 410-8, (2016) (PubMed).

Chen, Yang, Huang, Pan, Liu, Qiu: "Angiotensin-(1-7) attenuates lung fibrosis by way of Mas receptor in acute lung injury." in: The Journal of surgical research, Vol. 185, Issue 2, pp. 740-7, (2013) (PubMed).

Xie, Li, Wang, Zhang, Peng, Yang, Zou, Ge, Chen, Chen: "In vivo delivery of adenoviral vector containing interleukin-17 receptor a reduces cardiac remodeling and improves myocardial function in viral myocarditis leading to dilated cardiomyopathy." in: PLoS ONE, Vol. 8, Issue 8, pp. e72158, (2013) (PubMed).

Zhang, Shen, Zhang, Wan, Yao, Wu, Wang, Chen, Yan, Jiang: "Induction of thoracic aortic remodeling by endothelial-specific deletion of microRNA-21 in mice." in: PLoS ONE, Vol. 8, Issue 3, pp. e59002, (2013) (PubMed).

Target Details for COL3A1

Target: COL3A1 (Collagen, Type III, alpha 1 (COL3A1))

Abstract: COL3A1 Products

Synonyms: EDS4A antibody, AW550625 antibody, Col3a-1 antibody, MMS10-W antibody, Ms10w antibody, mKIAA4231 antibody, col3a1 antibody, collagen type III alpha 1 chain antibody, collagen, type III, alpha 1 antibody, collagen, type III, alpha 1 (Ehlers-Danlos syndrome type IV, autosomal dominant) S homeolog antibody, COL3A1 antibody, Col3a1 antibody, col3a1 antibody, col3a1.S antibody

Background: Collagen III is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with type I Collagen. Mutations in the gene are associated with Ehlers Danlos syndrome type IV, and with aortic and arterial aneurysms. Although alternate transcripts have been detected for this gene, they are the result of mutations, these mutations alter splicing, often leading to the exclusion of multiple exons.Synonyms: COL3A1

Molecular Weight: approx. 138 kDa

Gene ID: 1281

NCBI Accession: NP_000081

UniProt: P02461

Pathways: Autophagy, Growth Factor Binding