Aurora Kinase B antibody

Catalog No. ABIN272108

The Rabbit Polyclonal anti-Aurora Kinase B antibody is suitable to detect Aurora Kinase B in samples from Human, Mouse and Rat. It has been validated for WB, IF and IHC (p).

Quick Overview for Aurora Kinase B antibody (ABIN272108)

Target: Aurora Kinase B (AURKB)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aurora Kinase B antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Aurora Kinase B Antibody

Specificity: This antibody detects endogenous levels of AurB protein. (region surrounding Tyr8)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific Immunogen

Application Details

Application Notes: ELISA: 1/5000approx. 1/10000. Western Blot: 1/500approx. 1/1000. Immunohistochemistry: 1/50approx. 1/200. Immunofluorescence: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Aurora Kinase B

Target: Aurora Kinase B (AURKB)

Alternative Name: Aurora Kinase B

Background: Aurora kinases belong to a highly conserved family of mitotic serine/threonine kinases with three members identified among mammals: Aurora A, B, and C. Studies on the temporal expression pattern and subcellular localization of Aurora kinases in mitotic cells suggest an association with mitotic structure. Aurora kinase functional influences span from G2 phase to cytokinesis and may be involved in key cell cycle events such as centrosome duplication, chromosome bi-orientation and segregation, cleavage furrow positioning, and ingression.Synonyms: AIK2, AIM1, ARK2, AURKB, Aurora- and Ipl1-like midbody-associated protein 1, Aurora-B, Aurora/IPL1-related kinase 2, STK-1, STK12, Serine/threonine-protein kinase 12

Molecular Weight: approx. 39 kDa

Gene ID: 9212

NCBI Accession: NP_004208

UniProt: Q96GD4

Pathways: TCR Signaling, Cell Division Cycle, Maintenance of Protein Location, Hepatitis C, Toll-Like Receptors Cascades