BACH1 antibody

Catalog No. ABIN272133

Product Details anti-BACH1 Antibody

Target: BACH1 (BTB and CNC Homology 1, Basic Leucine Zipper Transcription Factor 1 (BACH1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BACH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of BACH1 protein. (region surrounding Leu160)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 20000approx. 140000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for BACH1

Target: BACH1 (BTB and CNC Homology 1, Basic Leucine Zipper Transcription Factor 1 (BACH1))

Alternative Name: BACH1 (BACH1 Products)

Synonyms: zgc:112205 antibody, BACH1 antibody, 6230421P05Rik antibody, AI323795 antibody, BACH-1 antibody, BTB domain and CNC homolog 1 antibody, BTB and CNC homology 1, basic leucine zipper transcription factor 1 b antibody, BTB and CNC homology 1, basic leucine zipper transcription factor 1 antibody, BACH1 antibody, bach1b antibody, bach1 antibody, Bach1 antibody

Background: Members of the small Maf family (MafK, MafF, and MafG) are basic region leucine zipper (bZip) proteins that can function as transcriptional activators or repressors. They dimerize with other proteins and bind DNA to either represse or activate transcription depending on the dimer compositions. BACH1 and BACH2, heterodimerization partners of MafK, are members of a novel family of BTB/POZ-basic region leucine zipper (bzip) factors. BACH1 and BACH2 have significant similarity to each other in BTB domain and Cap'n' collar-type bZip domain but are otherwise divergent. BACH1 appears ubiquitous, whereas BACH2 is restricted to monocytes, neuronal cells and is abundantly expressed in the early stages of B-cell differentiation. BACH2, a 841 amino acid polypeptide, is an Nrf2-related transcription repressor and a tissue-specific partner of the Maf oncoprotein family. In culture cells, BACH2 is localized to the cytoplasm through its C-terminal cytoplasmic localization signal (CLS). Oxidative stressors aborted the CLS activity and induce nuclear accumulation of BACH2, which mediates nucleocytoplasmic communciation to couple oxidative stress and transcription repression in mammalian cells. BACH2 heterodimerizes with MAZR through its BTB/POZ domain to activate transcription. BACH2 also plays an important role in the regulation of B cell development.Synonyms: BTB and CNC homolog 1, HA2303, Transcription regulator protein BACH1, basic leucine zipper transcription factor 1

Molecular Weight: approx. 83 kDa

Gene ID: 571

NCBI Accession: NP_001177

UniProt: O14867