FYN antibody (AA 240-290)

Catalog No. ABIN2746628

Product Details anti-FYN Antibody

Target: FYN (FYN Oncogene Related To SRC, FGR, YES (FYN))

Binding Specificity: AA 240-290

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FYN antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: The antibody labels a strong band of Fyn at 64 kDa in ABIN1741653 samples and in other cancer cell lines.

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): The antibody does not cross react to any other cellular protein.

Characteristics: Fyn selective antibodies were generated against a peptide taken from the human protein. The Fyn-selective antibodies are affinity purified on an immobilized antigen based affinity matrix, the isolated antibodies were then stabilized in antibody stabilization buffer for long-term storage. The Fyn-selective antibodies are fully characterized for applications in western blotting and ELISA at the recommended dilutions. The supplier provides Fyn Western blot positive control samples in ready-to-use SDS-PAGE sample buffer. Limited quantities of antigenic blocking peptide for Fyn antibody is also available, please inquire before ordering.

Purification: Affinity Purified

Immunogen: Synthetic peptide taken within amino acid region 240-290 on human Fyn protein.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Synonyms: Proto-oncogene c-Fyn, p59, Src-like-kinase, SLK, Protooncogene Syn.P59-Fyn

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.55-0.75 μg/μL

Handling Advice: Working solutions of antibodies in buffer should be filtered through 0.45 μm filter after every use for long-term storage.

Storage: -20 °C

Storage Comment: For long-term storage of keep at -20 °C. We don't recommend storage of very dilute antibody solutions unless they are prepared in specially formulated multi use antibody dilution buffer.

Target Details for FYN

Target: FYN (FYN Oncogene Related To SRC, FGR, YES (FYN))

Alternative Name: FYN (FYN Products)

Synonyms: im:6908851 antibody, si:dkey-119m7.5 antibody, zgc:109996 antibody, c-fyn antibody, cfyn-a antibody, SLK antibody, SYN antibody, p59-FYN antibody, PKC antibody, fyn antibody, zgc:86720 antibody, AI448320 antibody, AW552119 antibody, FYN proto-oncogene, Src family tyrosine kinase antibody, FYN proto-oncogene, Src family tyrosine kinase S homeolog antibody, FYN proto-oncogene, Src family tyrosine kinase b antibody, FYN proto-oncogene, Src family tyrosine kinase L homeolog antibody, FYN proto-oncogene, Src family tyrosine kinase a antibody, Fyn proto-oncogene antibody, FYN antibody, fyn.S antibody, fynb antibody, fyn.L antibody, Fyn antibody, fyna antibody

Background: Protein tyrosine kinases are large multigene families which are important mediators of the signaling transduction pathways, responsible for intracellular signal transduction. Tyrosine kinases are a diverse group of cell surface transmembrane proteins that act as receptors for cytokines, growth factors, hormones and other signaling molecules. Receptor tyrosine kinases are expressed in many cell types and play important roles in a wide variety of cellular processes, including growth, differentiation, angiogenesis, metabolism and apoptosis in response to external and internal stimuli. Over-expression and/or structural alteration of tyrosine kinase family members are associated to human cancers. Tumor cells use RTK transduction pathways to achieve tumor growth, angiogenesis and metastasis making it a target in approaches of cancer therapy. Fyn kinase is a 64 kDa protein and one of the 9 members of the Src family kinase (SFK) which are important in the regulation of growth and differentiation of eukaryotic cells. Src-family kinases transduce signals that are involved in the control of a variety of cellular processes, including proliferation, differentiation, motility, and adhesion. Synthesized and N-myristoylated Fyn protein is targeted to the plasma membrane, where it is palmitoylated. Fyn encodes a membraneassociated tyrosine kinase that is implicated in the control of cell growth and it plays a key role in T-cell antigen receptor (TCR) signaling. Dually acetylated Fyn clusters in caveolae-like membrane micro domains and interacts with a variety of other signaling molecules. Fyn's biological functions are diverse and include signaling via the T cell receptor, regulation of brain function and adhesion mediated signaling. It is required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. The human Fyn gene maps to chromosome 6q21 and encodes a 537 amino acid protein.

UniProt: P06241

Pathways: JAK-STAT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Activated T Cell Proliferation, Thromboxane A2 Receptor Signaling