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IKZF2 antibody (AA 51-107)

IKZF2 Reactivity: Human, Mouse FACS Host: Armenian Hamster Monoclonal 22F6 unconjugated
Catalog No. ABIN2749084
  • Target See all IKZF2 Antibodies
    IKZF2 (IKAROS Family Zinc Finger 2 (IKZF2))
    Binding Specificity
    • 15
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 51-107
    Reactivity
    • 39
    • 12
    • 9
    • 4
    • 4
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    Human, Mouse
    Host
    • 34
    • 2
    • 2
    • 1
    Armenian Hamster
    Clonality
    • 36
    • 3
    Monoclonal
    Conjugate
    • 23
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This IKZF2 antibody is un-conjugated
    Application
    • 35
    • 13
    • 13
    • 11
    • 10
    • 6
    • 6
    • 4
    • 3
    • 3
    • 3
    Flow Cytometry (FACS)
    Specificity
    The Armenian hamster monoclonal antibody 22F6 recognizes Helios, a transcription factor (intracellular antigen) expressed in some hematopoietic stem cells, and at high levels in thymic-derived regulatory T cells. The epitope is located between amino acids 51 and 107.
    Cross-Reactivity (Details)
    Human, Mouse
    Purification
    Purified by protein-A affinity chromatography.
    Purity
    > 95 % (by SDS-PAGE)
    Immunogen
    Peptide coresponding to the amino acids 51-107 of Helios
    Clone
    22F6
    Isotype
    IgG
    Top Product
    Discover our top product IKZF2 Primary Antibody
  • Application Notes
    Flow cytometry: Recommended dilution: 1-4 μg/mL.,Recommended protocol: 1) Perform staining of cell surface markers (CD25, CD4 etc.) for 20 min. at room temperature in the dark, 100 μL of peripheral blood. 2) Add 3 mL of PBS with 1 % BSA, centrifugate at 300g and discard the supernatant. Further steps perform on ice and with ice-cold reagents. 3) Resuspend the cells in 5 mL of cold fixation solution (Miltenyi Biotec) and incubate for 30 min. on ice. 4) Centrifugate for 5 min. at 1000 g, 4 °C, and discard the supernatant. 5) Resuspend the cells in 5 mL of ice-cold PBS with 1 % BSA. 6) Centrifugate for 5 min. at 1000 g, 4 °C, and discard the supernatant. 7) Resuspend the cells in 5 mL of ice-cold permeabilization solution (Miltenyi Biotec) and incubate 5 min. 8) Centrifugate for 5 min. at 1000 g, 4 °C, and discard the supernatant. 9) Resuspend the cells in μl of ice-cold permeabilization solution and add 20 μL of FcR blocking solution and incubate for 5 min. (4 °C, in the dark). 10) Perform intracellular staining of Helios for 30 min. (4 °C, in the dark) with 10 μL of anti-Helios PE antibody (22F6) in 100 μL. 11) Add 2 mL of ice-cold permeabilization solution and incubate for 5 min. at 4 °C in the dark. 12) Centrifugate for 5 min. at 1000 g, 4 °C, and discard the supernatant. 13) Resuspend the cells in 3 mL of ice-cold PBS with 1 % BSA. 14) Centrifugate for 5 min. at 1000 g, 4 °C, and discard the supernatant. 15) Resuspend the cells in 150 μL of ice-cold PBS with 1 % BSA and measure on a flow cytometry device with appropriate setting as soon as possible. Keep cold until measuring.
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Do not freeze.
  • Blankenhaus, Reitz, Brenz, Eschbach, Hartmann, Haben, Sparwasser, Huehn, Kühl, Feyerabend, Rodewald, Breloer: "Foxp3? regulatory T cells delay expulsion of intestinal nematodes by suppression of IL-9-driven mast cell activation in BALB/c but not in C57BL/6 mice." in: PLoS pathogens, Vol. 10, Issue 2, pp. e1003913, (2014) (PubMed).

    McIver, Melenhorst, Wu, Grim, Ito, Cho, Hensel, Battiwalla, Barrett: "Donor lymphocyte count and thymic activity predict lymphocyte recovery and outcomes after matched-sibling hematopoietic stem cell transplant." in: Haematologica, Vol. 98, Issue 3, pp. 346-52, (2013) (PubMed).

    Serre, Bénézech, Desanti, Bobat, Toellner, Bird, Chan, Kastner, Cunningham, Maclennan, Mohr: "Helios is associated with CD4 T cells differentiating to T helper 2 and follicular helper T cells in vivo independently of Foxp3 expression." in: PLoS ONE, Vol. 6, Issue 6, pp. e20731, (2011) (PubMed).

    Pinheiro, Singh, Grant, Appleton, Sacchini, Walker, Chadbourne, Palmer, Armitage-Chan, Thompson, Williamson, Cunningham, Garden: "Phenotypic and functional characterization of a CD4(+) CD25(high) FOXP3(high) regulatory T-cell population in the dog." in: Immunology, Vol. 132, Issue 1, pp. 111-22, (2010) (PubMed).

  • Target
    IKZF2 (IKAROS Family Zinc Finger 2 (IKZF2))
    Alternative Name
    Helios (IKZF2 Products)
    Synonyms
    ANF1A2 antibody, HELIOS antibody, ZNF1A2 antibody, ZNFN1A2 antibody, helios antibody, znf1a2 antibody, znfn1a1 antibody, znfn1a2 antibody, IKZF2 antibody, MGC159581 antibody, DKFZp469N0319 antibody, A730095J18Rik antibody, Helios antibody, Zfpn1a2 antibody, Znfn1a2 antibody, ikzf2 antibody, znfn1a1-a antibody, IKAROS family zinc finger 2 antibody, si:zfos-1425h8.1 antibody, IKAROS family zinc finger 2 (Helios) antibody, IKAROS family zinc finger 2 L homeolog antibody, IKZF2 antibody, ikzf2 antibody, Ikzf2 antibody, si:zfos-1425h8.1 antibody, ikzf2.L antibody
    Background
    IKAROS family zinc finger 2,Helios, also known as IKZF2 (Ikaros family zinc finger protein 2) is a hematopoietic-specific transcription factor involved in the regulation of lymphocyte development, together with other members of this family, such as Aiolos and Ikaros. Helios forms homo- and heterodimers with these proteins and is thought to function predominantly in early hematopoietic development. Expression of Helios, Aiolos and Ikaros is restricted to cells of the hematopoietic system, whereas other family members, Eos and Pegassus, are more widely expressed. Helios is expressed at early stages of thymocyte development. In mature T cells, Helios has been strongly associated with Treg cells.,ANF1A2, IKZF2
    Gene ID
    22807
    UniProt
    Q9UKS7
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