CD137 antibody (Extracellular Domain) (PE)

Catalog No. ABIN2749106

This Mouse Monoclonal antibody specifically detects CD137 in FACS. It exhibits reactivity toward Human and Non-Human Primate. It has been mentioned in 4+ publications

Quick Overview for CD137 antibody (Extracellular Domain) (PE) (ABIN2749106)

Target: CD137 (TNFRSF9) (Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9))

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD137 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: 4B4-1

Product Details anti-CD137 Antibody (PE)

Binding Specificity: Extracellular Domain

Purpose: Anti-Hu CD137 PE

Cross-Reactivity (Details): Human, Non-Human Primates

Characteristics: The mouse monoclonal antibody 4B4-1 recognizes an extracellular conformational epitope on CD137, an approximately 40 kDa type I transmembrane protein of the TNFR family expressed mainly on activated T cells.

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Recombinant human CD137 ectodomain

Isotype: IgG1, kappa

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze,Protect from prolonged exposure to light

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-CD137 antibody (PE) (ABIN2749106)

Yi, Zhao, Wang, Dai, Hellström, Hellström, Zhang: "Human and mouse CD137 have predominantly different binding CRDs to their respective ligands." in: PLoS ONE, Vol. 9, Issue 1, pp. e86337, (2014) (PubMed).

Bellarosa, Bressan, Bigioni, Parlani, Maggi, Binaschi: "SAHA/Vorinostat induces the expression of the CD137 receptor/ligand system and enhances apoptosis mediated by soluble CD137 receptor in a human breast cancer cell line." in: International journal of oncology, Vol. 41, Issue 4, pp. 1486-94, (2013) (PubMed).

Fernández Do Porto, Jurado, Pasquinelli, Alvarez, Aspera, Musella, García: "CD137 differentially regulates innate and adaptive immunity against Mycobacterium tuberculosis." in: Immunology and cell biology, Vol. 90, Issue 4, pp. 449-56, (2012) (PubMed).

Chan, Voskens, Lin, Schindler, Azimzadeh, Wang, Taylor, Strome, Schulze: "Epitope mapping of a chimeric CD137 mAb: a necessary step for assessing the biologic relevance of non-human primate models." in: Journal of molecular recognition : JMR, Vol. 22, Issue 3, pp. 242-9, (2009) (PubMed).

Target Details for CD137

Target: CD137 (TNFRSF9) (Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9))

Alternative Name: CD137

Background: TNF receptor superfamily member 9,CD137, also known as TNFRSF9 or 4-1BB, is an inducible costimulatory molecule expressed mainly on activated T cells. Its ligand, known as 4-1BBL, is expressed on activated macrophages, mature B cells, hematopoietic stem cells, and myeloid progenitor cells. CD137 signaling leads to maintaining the survival of activated T cells and CD8+ memory T cells, and clonal expansion of T cells, but also to suppressing myelopoiesis and dendritic cell development. Triggered CD137 induces a cytokine release profile regulating peripheral monocyte survival. Soluble forms of CD137 may provide negative control mechanism for some immune responses.,TNFRSF9, 41BB, 4-1BB, MGC2172

Gene ID: 3604

UniProt: Q07011

Pathways: Cancer Immune Checkpoints