CD94 antibody (FITC)

Catalog No. ABIN2749143

Product Details anti-CD94 Antibody (FITC)

Target: CD94 (KLRD1) (Killer Cell Lectin-Like Receptor Subfamily D, Member 1 (KLRD1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD94 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Specificity: The mouse monoclonal antibody HP-3D9 recognizes an extracellular epitope of CD94, a 70 kDa type II transmembrane glycoprotein expressed on NK cells, NK-T cells, and subsets of CD8+ T cells and gamma/delta T cells.

Cross-Reactivity (Details): Human

Purification: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Cultured human NK cells

Clone: HP-3D9

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Comment:

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Handling

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-CD94 antibody (FITC) (ABIN2749143)

Yu, Mao, Wei, Hughes, Zhang, Park, Liu, McClory, Marcucci, Trotta, Caligiuri: "CD94 surface density identifies a functional intermediary between the CD56bright and CD56dim human NK-cell subsets." in: Blood, Vol. 115, Issue 2, pp. 274-81, (2010) (PubMed).

Hallermalm, Seki, De Geer, Motyka, Bleackley, Jager, Froelich, Kiessling, Levitsky, Levitskaya: "Modulation of the tumor cell phenotype by IFN-gamma results in resistance of uveal melanoma cells to granule-mediated lysis by cytotoxic lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 6, pp. 3766-74, (2008) (PubMed).

Bovenschen, Van De Kerkhof, Gerritsen, Seyger: "The role of lesional T cells in recalcitrant psoriasis during infliximab therapy." in: European journal of dermatology : EJD, Vol. 15, Issue 6, pp. 454-8, (2005) (PubMed).

Wada, Matsumoto, Maenaka, Suzuki, Yamamoto: "The inhibitory NK cell receptor CD94/NKG2A and the activating receptor CD94/NKG2C bind the top of HLA-E through mostly shared but partly distinct sets of HLA-E residues." in: European journal of immunology, Vol. 34, Issue 1, pp. 81-90, (2004) (PubMed).

Seo, Tokura, Ishihara, Takeoka, Tagawa, Takigawa: "Disordered expression of inhibitory receptors on the NK1-type natural killer (NK) leukaemic cells from patients with hypersensitivity to mosquito bites." in: Clinical and experimental immunology, Vol. 120, Issue 3, pp. 413-9, (2000) (PubMed).

Phillips, Chang, Mattson, Gumperz, Parham, Lanier: "CD94 and a novel associated protein (94AP) form a NK cell receptor involved in the recognition of HLA-A, HLA-B, and HLA-C allotypes." in: Immunity, Vol. 5, Issue 2, pp. 163-72, (1996) (PubMed).

Target Details for CD94

Target: CD94 (KLRD1) (Killer Cell Lectin-Like Receptor Subfamily D, Member 1 (KLRD1))

Alternative Name: CD94 (KLRD1 Products)

Synonyms: CD94 antibody, CD94-B antibody, Cd94 antibody, killer cell lectin like receptor D1 antibody, killer cell lectin-like receptor subfamily D, member 1 antibody, killer cell lectin-like receptor, subfamily D, member 1 antibody, KLRD1 antibody, Klrd1 antibody

Background: Killer cell lectin like receptor D1,CD94, also known as KLRD1 (killer cell lectin-like receptor D1), is a transmembrane glycoprotein of the C-type lectin family, which forms disulfide-linked heterodimers with NKG2A, B, C, E, H proteins, constituting functionally distinct receptors of NK cells and related cell types. CD94/NKG2A and CD94/NKG2B heterodimers serve as inhibitory, whereas CD94/NKG2C and CD94/NKG2E as activating receptors. The ligand for CD94/NKG2 complexes has been identified as HLA-E. Extent of CD94 expression on NK cell surface can be used to demonstrate their progress through the differentiation process.,KLR-D1, KLRD1, KCLLRD1

Gene ID: 3824

UniProt: Q13241